We narrowed to 58,555 results for: tra

1. Tol2_B-act_dCas9-Vp64-T2A-Citrine

   Plasmid

   #200248

   Purpose

   Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   dCas9-Vp64-T2A-Citrine
   

   Use

   Zebrafish expression

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPF215

   Plasmid

   #106377

   Purpose

   C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

   Insert

   Ptet-himar1-Ter(slpA)-transposon
   

   Use

   E. coli - c. difficile shuttle vector

   Mutation

   Codon optimised for C. difficile

   Promoter

   Ptet

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL4393 (pClpX_Eco)

   Plasmid

   #200907

   Purpose

   Expresses human codon-optimized E. coli ClpX from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   EcoClpX
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-DDX4N-mCh-Cry2WT

   Plasmid

   #101225

   Purpose

   DDX4(1-236) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   DDX4(1-236)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL3713 (pTnsC_Pse)

   Plasmid

   #200900

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsC
   

   Tags

   3xNLS

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RabV CVS-N2c(deltaG)-mCherry

   Plasmid

   #73464

   Purpose

   Expresses mCherry for monosynaptic circuit mapping

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   mCherry
   

   Use

   Neurotropic virus

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH761-CEN-mCherry_v4

   Plasmid

   #40599

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
   

   Expression

   Yeast

   Mutation

   mCherry ORF partially codon optimised for yeast e…

   Promoter

   GPD (TDH3)

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

   Plasmid

   #209666

   Purpose

   Non-responsive control

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   cpSFGFP.HaloTag
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTnsABC

   Plasmid

   #160031

   Purpose

   Expresses V. cholerae TnsABC

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   VchTnsA, VchTnsB, VchTnsC
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Mutation

   no

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. RabV CVS-N2c(deltaG)-FlpO

    Plasmid

    #73473

    Purpose

    Expresses FlpO for circuit mapping and genetic manipulation

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    FlpO
    

    Use

    Neurotropic virus

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PBCAG-EGFR

    Plasmid

    #40974

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Insert

    EGFR (EGFR Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. IVT-R2Tg_opt

    Plasmid

    #223247

    Purpose

    In vitro transcription of the optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Use

    In vitro transcription

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mt POLRMT

    Plasmid

    #205059

    Purpose

    POLRMT protein expression

    Depositor

    Smita Patel

    Article

    Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

    Insert

    POLRMT (POLRMT Human)
    

    Expression

    Bacterial

    Mutation

    Codon optimized

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM60-Ptb

    Plasmid

    #38245

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Ptb (Ptbp1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMDJ231 - Phsp Cas9

    Plasmid

    #191382

    Purpose

    Heat shock inducible Cas9 expression in C. elegans.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Phsp-16.41 Cas9 gpd-2 tagRFP-t
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    Phsp-16.41

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL0283 (pTnsABC)

    Plasmid

    #130633

    Purpose

    Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1-delta30-TFEB

    Plasmid

    #44445

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-30; H32D

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. RNA-Protein Landing Pad

    Plasmid

    #226162

    Purpose

    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    

    Use

    Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDule-Tet3.0

    Plasmid

    #174080

    Purpose

    Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    Tet3.0 tRNA synthetase and tRNA
    

    Expression

    Bacterial

    Mutation

    L270G, Y271L, N311G, C313A

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLuc-CDS

    Plasmid

    #42100

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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