We narrowed to 161,570 results for: addgene

1. OmEF1aCas9P2ApuroSB

   Plasmid

   #165484

   Purpose

   Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

   Depositor

   Dietmar Kueltz

   Article

   Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

   Insert

   Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mutation

   Promoter

   Oreochromis mossambicus EF1 alpha promoter (OmEF1…

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)

   Plasmid

   #110561

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-aacC1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhaSR-PrhaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ShuttleMCP

   Plasmid

   #50959

   Purpose

   A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter

   Depositor

   James Bamburg

   Article

   Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   uses portion of the mouse cofilin gene as a mouse…

   Available since

   Feb. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pX330-sgRNA_Dicer_2

   Plasmid

   #68808

   Purpose

   specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

   Depositor

   Constance Ciaudo

   Article

   Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

   Insert

   sgRNA mouse Dicer1
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG1-puro-sgATL3-2

   Plasmid

   #109010

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3-sgRNA #2 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLG1-puro-sgATL3-5

   Plasmid

   #109011

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3-sgRNA #5 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. attB53-Pac-tetO-mCherry-attB53

   Plasmid

   #183612

   Purpose

   RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   tetO-mCherry
   

   Use

   Rmce

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

   Plasmid

   #216668

   Purpose

   Expresses membrane-bound mCherry in mammalian cells

   Depositor

   Leonardo Morsut

   Insert

   mCherry
   

   Use

   Tags

   PDGFR Transmembrane Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_EF1a_flagLaM4-Notch-Gal4VP64

   Plasmid

   #216669

   Purpose

   Expresses anti-mCherry synNotch Gal4VP64

   Depositor

   Leonardo Morsut

   Insert

   anti-mCherry synNotch Gal4VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-eCas9-GFP-U6-gRNA

    Plasmid

    #79145

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells

    Depositor

    Jizhong Zou

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJV53

    Plasmid

    #26904

    Depositor

    Graham Hatfull

    Article

    van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

    Insert

    Che9c genes 60-61
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

    Plasmid

    #175574

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. integrin beta6 pBabe puro

    Plasmid

    #13596

    Depositor

    Dean Sheppard

    Insert

    integrin beta 6 (ITGB6 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCMV_GFP_24

    Plasmid

    #238190

    Purpose

    Empty lentiviral vector for expressing protein sequences fused to GFP and FTH1 (24-mer), under a CMV promoter

    Depositor

    Feng Zhang

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP-FTH1 and SV40

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. SRPK1 (SRPK1A-c100)

    Plasmid

    #98246

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SRPK1A (SRPK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p14-9VN

    Plasmid

    #50930

    Purpose

    Expresses human SRP14 and SRP9 proteins as a fusion protein in mammalian cells.

    Depositor

    Katharina Strub

    Insert

    SRP14 and SRP9 (SRP14, SRP9 Human)
    

    Use

    Tags

    VN tag and flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-SAGFP

    Plasmid

    #127100

    Purpose

    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    SA-GFP reporter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. XLone-eGFP NLS (no drug resistant gene)

    Plasmid

    #140032

    Purpose

    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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