We narrowed to 1,891 results for: ogn

1. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   TRC

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Stat3 Flag pRc/CMV

   Plasmid

   #8707

   Depositor

   Jim Darnell

   Article

   Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   *see comments

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221_HUWE1_isoform1

   Plasmid

   #190464

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_NR3C2_Hormone-recep

   Plasmid

   #109848

   Purpose

   Protein expression and purification of NR3C2_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   NR3C2_Hormone-recep (NR3C2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINB-AMSH*

   Plasmid

   #66712

   Purpose

   Expresses activated AMSH in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   hbp (Stam2 Mouse)
   AMSH (STAMBP Human)
   

   Use

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCS2 GFP-GSK3mut-MAPK

   Plasmid

   #29690

   Purpose

   GFP reporter protein fused to three mutated GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
   

   Use

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site, GSK3 …

   Promoter

   SP6

   Available since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_RANBP2_Ran-BP2

   Plasmid

   #109839

   Purpose

   Protein expression and purification of RANBP2_Ran-BP2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RANBP2_Ran-BP2 (RANBP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. iSpyMac

   Plasmid

   #155014

   Purpose

   Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

   Insert

   iSpyMac
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PID grafted onto SpyCas9 N-terminal domain, R221K…

   Promoter

   EF1ɑ

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-TYMS

   Plasmid

   #100545

   Purpose

   mammalian expression of thymidylate synthetase

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

   Insert

   thymidylate synthetase (TYMS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

    Plasmid

    #177841

    Purpose

    Mammalian Expression of Folate Receptor

    Depositor

    Karsten Melcher

    Article

    Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

    Insert

    Folate receptor alpha (FOLR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    all 11 C mutated to S

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-PCNA-IRES-puro2b

    Plasmid

    #26461

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    pEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOPINB-OTUB1*

    Plasmid

    #65441

    Purpose

    Expresses activated OTUB1 in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    UbcH5b (UBE2D2 Human)
    otubain-1 (OTUB1 )
    

    Use

    Tags

    His-3C

    Expression

    Bacterial

    Mutation

    Active site cysteine mutated to alanine

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

    Plasmid

    #60815

    Purpose

    dCas9 with single cysteine residue (M1C) for site-specific labelling

    Depositor

    Jennifer Doudna

    Article

    O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

    Insert

    dCas9 M1C D10A C80S H840A C574S
    

    Use

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    M1C D10A C80S H840A C574S

    Promoter

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-GST-His6-1_HBD

    Plasmid

    #171869

    Purpose

    Expresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Hybrid binding domain of Rnase H1
    

    Use

    Tags

    GST- and His10-tag

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-WIPI4

    Plasmid

    #42528

    Depositor

    James Hurley

    Article

    Baskaran et al Mol Cell. 2012 Aug 10;47(3):339-48. doi:

    Insert

    WIPI4 (WDR45 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET-Galectin-8 FL

    Plasmid

    #68805

    Purpose

    Express Galectin-8 FL protein in Bacterial cells

    Depositor

    Hyun Kyu Song

    Article

    Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

    Insert

    Galectin-8 (LGALS8 Human)
    

    Use

    Tags

    N-terminal 6-histidine-tag

    Expression

    Bacterial

    Mutation

    No mutation

    Promoter

    T7

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pHH0103_NR1H2_Hormone-recep

    Plasmid

    #109827

    Purpose

    Protein expression and purification of NR1H2_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR1H2_Hormone-recep (NR1H2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-TK1

    Plasmid

    #100544

    Purpose

    mammalian expression of thymidine kinase 1

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

    Insert

    thymidine kinase 1 (TK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-Sig10-Fc-IG

    Plasmid

    #183722

    Purpose

    Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

    Insert

    Siglec-10
    

    Use

    Retroviral

    Tags

    Fc of human IgG1

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pESUMO-hRORg1

    Plasmid

    #170330

    Purpose

    Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

    Depositor

    Patrick Griffin

    Article

    Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

    Insert

    RORG isoform 1 (RORC Human)
    

    Use

    Tags

    HisSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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