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Showing: 811 - 840 of 880 results

  1. pcDNA3.1(+)-AKAP95-FRB

    Plasmid
    #138254
    Purpose
    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Article
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Insert
    AKAP95-FRB (AKAP8 Human)
    Use
    Tags
    FRB rapamycin binding domain and HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    138254-plasmid-map-sequence-id-307498

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  2. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid
    #140163
    Purpose
    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker
    Depositor
    Benjamin Glick
    Article
    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.
    Insert
    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
    Use
    Tags
    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140163-plasmid-map-sequence-id-275937

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  12. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid
    #193719
    Purpose
    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Synthetic, Human)
    Use
    Tags
    2xHA-FRB
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193719-plasmid-map-sequence-id-383790

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  13. pcDNA_DiCre2_60.F2

    Plasmid
    #60210
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 60-343 …
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60210-plasmid-map-sequence-id-96296

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  14. pcDNA_DiCre2_59.F2

    Plasmid
    #60209
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 19-59 o…
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60209-plasmid-map-sequence-id-96295

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  15. pAGH50

    Plasmid
    #107254
    Purpose
    Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.
    Depositor
    Erin O'Shea
    Article
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    vipp1-gfp-fkbp12
    Use
    Suicide vector for destined for double homologous…
    Tags
    GFP and FKBP12
    Expression
    Bacterial
    Mutation
    Promoter
    native
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107254-plasmid-map-sequence-id-214328

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  27. pJD427

    Plasmid
    #154371
    Purpose
    Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters
    Depositor
    Joseph Davis
    Article
    Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13
    Insert
    proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    154371-plasmid-map-sequence-id-311827

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Showing: 811 - 840 of 880 results