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Plasmid#164837PurposeExpression of human talin2 head mutantDepositorInsertTLN2 (TLN2 Human)
UseTagsHis8ExpressionBacterialMutationLipid binding deficient mutationsPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-GFP-tglG
Plasmid#134679PurposeExpresses His6-GFP-TglG fusion in E. ColiDepositorInsertTglG
UseTagsHis6-GFPExpressionBacterialMutationPromoterT7Available sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a HAT epitope
Plasmid#129445Purposeexpression of HAT epitopeDepositorInsertHAT epitope
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a SznF E281A
Plasmid#139591Purposeexpresses SznF E281ADepositorInsertSznF E281A
UseTagshexahistidineExpressionBacterialMutationchanged glutamate 281 to alaninePromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a SznF H225A
Plasmid#139590Purposeexpresses SznF H225ADepositorInsertSznF H225A
UseTagshexahistidineExpressionBacterialMutationchanged histidine 225 to alaninePromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SznF E215A
Plasmid#139565Purposeexpresses SznF E215A in E. coliDepositorInsertSznF E215A
UseTagshexahistidineExpressionBacterialMutationchanged glutamate 215 to alaninePromoterT7Available sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-NT-G46D
Plasmid#134588PurposeExpress human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coliDepositorInsertDDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (G154D)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-FL-G84D
Plasmid#134577PurposeExpress human DDX43 mutant (full-length with G84D mutation) in E. coliDepositorInsertDDX43-G84D (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (G84D)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Loop
Plasmid#124551PurposeChimeric ETS domain: Loop of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 216 to 223 replaced with corresponding s…PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes7/Imdes7
Plasmid#107115Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des7DepositorInsertcolEdes7/Imdes7
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes8/Imdes8
Plasmid#107116Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des8DepositorInsertcolEdes8/Imdes8
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes9/Imdes9
Plasmid#107117Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des9DepositorInsertcolEdes9/Imdes9
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes10/Imdes10
Plasmid#107118Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des10DepositorInsertcolEdes10/Imdes10
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes11/Imdes11
Plasmid#107119Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des11DepositorInsertcolEdes11/Imdes11
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes12/Imdes12
Plasmid#107120Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des12DepositorInsertcolEdes12/Imdes12
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes13/Imdes13
Plasmid#107121Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des13DepositorInsertcolEdes13/Imdes13
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes14/Imdes14
Plasmid#107122Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des14DepositorInsertcolEdes14/Imdes14
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes15/Imdes15
Plasmid#107123Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des15DepositorInsertcolEdes15/Imdes15
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes16/Imdes16
Plasmid#107124Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des16DepositorInsertcolEdes16/Imdes16
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes17/Imdes17
Plasmid#107125Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des17DepositorInsertcolEdes17/Imdes17
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes18/Imdes18
Plasmid#107126Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des18DepositorInsertcolEdes18/Imdes18
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes1/Imdes1
Plasmid#107108Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des1DepositorInsertcolEdes1/Imdes1
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes2/Imdes2
Plasmid#107109Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des2DepositorInsertcolEdes2/Imdes2
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes3/Imdes3
Plasmid#107110Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des3DepositorInsertcolEdes3/Imdes3
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes3.5/Imdes3.5
Plasmid#107111Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5DepositorInsertcolEdes3.5/Imdes3.5
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes4/Imdes4
Plasmid#107112Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des4DepositorInsertcolEdes4/Imdes4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes5/Imdes5
Plasmid#107113Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des5DepositorInsertcolEdes5/Imdes5
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEdes6/Imdes6
Plasmid#107114Purposeplasmid for expression of computationally designed colicin endonuclease and immunity pair, des6DepositorInsertcolEdes6/Imdes6
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbI S178A
Plasmid#114159PurposeExpresses ClbI S178ADepositorInsertclbI
UseTags6xHis and 6xHis-Thrombin siteExpressionBacterialMutationMutated S178 to AlaPromoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbH-C-A2-PCP
Plasmid#114157PurposeExpresses ClbH-C-A2-PCPDepositorInsertclbH
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbHPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
UseTags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
UseTags6His-TEVExpressionBacterialMutationR47APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47K)
Plasmid#112723PurposeArg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.DepositorInsert6His-TEV-TBP6.7(R47K)
UseTags6His-TEVExpressionBacterialMutationR47KPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48A)
Plasmid#112724PurposeGln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.DepositorInsert6His-TEV-TBP6.7(Q48A)
UseTags6His-TEVExpressionBacterialMutationQ48APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
UseTags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(T50A)
Plasmid#112727PurposeLav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.DepositorInsert6His-TEV-TBP6.7(T50A)
UseTags6His-TEVExpressionBacterialMutationT50APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
UseTags6His-TEVExpressionBacterialMutationP51APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
UseTags6His-TEVExpressionBacterialMutationR52APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
UseTags6His-TEVExpressionBacterialMutationQ54APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_T1-NHis
Plasmid#109259PurposeNRPS T domain with N-terminal 6His tagDepositorArticleInsertRupA_T1
UseTagsHis tagExpressionBacterialMutationcontains aa 917..1025 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_T2-R-CHis
Plasmid#109258PurposeNRPS di-domain containing TR domains with C-terminal 6His tagDepositorArticleInsertRupA_T2-R
UseTagsHis tag and S-TagExpressionBacterialMutationcontains aa 1939..2443 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_R-NHis
Plasmid#109257PurposeNRPS R domain with N-terminal 6His tagDepositorArticleInsertRupA_R
UseTagsHis tagExpressionBacterialMutationcontains aa 2038..2443 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_C2-A2-T2-R-CHis
Plasmid#109256PurposeNRPS module containing CATR domains with C-terminal 6His tagDepositorArticleInsertRupA_C2-A2-T2-R
UseTagsHis tag and S-TagExpressionBacterialMutationcontains aa 1023..2443 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-RupA_C1-A1-T1-CHis
Plasmid#109255PurposeNRPS module containing CAT domains with C-terminal 6His tagDepositorArticleInsertRupA_C1-A1-T1
UseTagsHis tag and S-TagExpressionBacterialMutationcontains aa 1..1025 of WP_015523502.1PromoterT7Available sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX D144L
Plasmid#111514PurposeExpresses E. coli ClpX with a C-terminal His tag and a mutation from D144 to L144DepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationAspartatic acid 144 to LeucinePromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-1xTS
Plasmid#109418PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to one tyrosine sulfation (TS) motif. VHH-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP
Plasmid#110828PurposeBacterial expression of E.coli DHFR N23PP mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)C
Plasmid#108194PurposeExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C)-P450cam fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only