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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Plasmid

#194755

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Plasmid

#194753

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194752

Purpose

Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSK+ chickBMPR-1B CA

Plasmid

#49528

Purpose

cloning vector for constitutively active chick BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

Insert

BMPR-1B CA (BMPR1B Chicken)

Use

Tags

Expression

Bacterial

Mutation

constitutively active mutation (Q203D); also P2A,…

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pET15b/GgVcl V1-258 (aka VD1)

Plasmid

#46173

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-258 (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

aa 1-258 (VD1)

Promoter

T7

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pBS YBX1

Plasmid

#19217

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

YBX1 (YBX1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pIR3DH8K

Plasmid

#165069

Purpose

Knocking out GAL80 using KanMX4 marker

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-TPI1-KanMX4-gal80Arm2

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJET-LB-geneticin

Plasmid

#213468

Purpose

Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Left border of the TSI locus 1-split fragment of geneticin cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b/GgVcl V1-851 (aka Vh)

Plasmid

#46172

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-851 (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

aa 1-851 (aka Vh) head domain

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAL725

Plasmid

#119862

Purpose

precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)

Use

Tags

none

Expression

Bacterial

Mutation

Leucine 244 to Cysteine, Aspartate 287 to Cysteine

Promoter

T5

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMK287 (mAID-Hygro)

Plasmid

#72825

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Hygro

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMK288 (mAID-Bsr)

Plasmid

#72826

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Bsr

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMK281 (mCherry2-Hygro)

Plasmid

#72797

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Hygro

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS9

Plasmid

#111765

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMK279 (mClover-Bsr)

Plasmid

#72795

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Bsr

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS7

Plasmid

#111764

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK278 (mClover-Hygro)

Plasmid

#72794

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Hygro

Use

CRISPR

Tags

mClover

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
VinTS-tCRMod-GGSGGS5

Plasmid

#111763

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK282 (mCherry2-Bsr)

Plasmid

#72798

Purpose

C-terminal tagging with mCherry2 using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mCherry2-Bsr

Use

CRISPR

Tags

mCherry2

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pBS Calnexin

Plasmid

#19208

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Calnexin (CANX Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only