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pMiniT:SEP-GluA2 TKIT donor

Plasmid

#169421

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMD19T-slr0230-PL22-sgRNANT1-KmR

Plasmid

#73224

Purpose

Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

PL22-sgRNA NT1

Expression

Bacterial

Promoter

PL22

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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EC64434

Plasmid

#209518

Purpose

Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMS10

Plasmid

#230074

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mNeonGreen fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG and mNeonGreen

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMS02

Plasmid

#230066

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Article

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

UBC

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-GFPnls

Plasmid

#121938

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

PCP-GFPnls

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET19b+H3

Plasmid

#157807

Purpose

Expression of cysteine-free human histone H3 (c111A)

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H3

Expression

Bacterial

Mutation

C111A

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0mut

Plasmid

#153290

Purpose

Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in astrocytes

Depositor

Yulong Li

Article

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Insert

Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)

Use

AAV

Promoter

GfaABC1D

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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HSA1_His

Plasmid

#202763

Purpose

human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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STR-KDEL_PSAP-SBP-mCherry

Plasmid

#180440

Purpose

Co-expresses (via IRES) streptavidin-KDEL and Prosaposin-SBP-mCherry

Depositor

Shawn Ferguson

Article

Devireddy et al J Cell Biol. 2022 Feb 7;221(2):e20210404

Insert

Prosaposin (PSAP )

Tags

SBP-mCherry

Expression

Mammalian

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAG1188 mCherry-cagedFIREtag

Plasmid

#205177

Purpose

Expresses mCherry-cagedFIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag-DEVDG-MoA

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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EC70190

Plasmid

#209451

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

HvU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EC70196

Plasmid

#209457

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU3

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLCS110

Plasmid

#209331

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mStayGold fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAG1344 EGFP-FIREtag

Plasmid

#205165

Purpose

Expresses EGFP-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

EGFP-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-8XMS2

Plasmid

#121939

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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Thy1.1-GFP

Plasmid

#131703

Purpose

encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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EC70203

Plasmid

#209464

Purpose

Cloning protospacers for Cas9 editing

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

Cas9 sgRNA

Use

CRISPR

Promoter

TaU6

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-tCorn

Plasmid

#106233

Purpose

Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEAK10-His-Myc-Kal12

Plasmid

#25442

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal12

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-Sirius-8XPP7

Plasmid

#121943

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHelper_TetR/pTet-Tnpase (camR)

Plasmid

#213910

Purpose

ATc-inducible himar1C9 transposase expression - cam resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

TetR/ptet ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CaM-V5-sTb(N)

Plasmid

#219783

Purpose

sTb(N) half of CaST for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CaM-V5-sTb(N)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

Synapsin

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEAK10-His-Myc-Kal9

Plasmid

#25441

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal9

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pEAK10-His-Myc-Kal5

Plasmid

#25440

Depositor

Betty Eipper

Article

Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

Insert

Kal5

Tags

His and Myc

Expression

Mammalian

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pERB221

Plasmid

#61502

Purpose

const. CenpB-GFP-Halo + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

CenpB-GFP-Halo
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-HxB.L

Plasmid

#65414

Purpose

contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

Depositor

Harold Erickson

Article

Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

Insert

TNC large splice variant (TNC Human)

Expression

Bacterial

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
His-Tev-UbE2D3

Plasmid

#186805

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin conjugating enzyme His-Tev-UbE2D3.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-UbE2D3 (UBE2D3 Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-QUASM1

Plasmid

#155114

Purpose

5' entry vector containing QUASM1 promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1

Use

Gateway 5' entry vector

Promoter

QUASM1-E1b

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-GFP-W

Plasmid

#196046

Purpose

lentiviral expression of the green fluorescent protein driven by cytomegalovirus virus promoter

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCS109

Plasmid

#209330

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mTagBFP2 fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG1237 FIREmate-ECFP-Fis1

Plasmid

#205173

Purpose

Expresses FIREmate-ECFP-Fis1 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Fis1

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_36_QUEEN-2m

Plasmid

#177708

Purpose

Plasmid for genome integration in X2 site expressing QUEEN-2m (ATP biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-QUEEN2m

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN1 TKIT donor

Plasmid

#169427

Purpose

Donor DNA for TKIT knockin of SEP to NR1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 52,767 results for: son