We narrowed to 13,804 results for: TIM

1. RIPK2 gRNA (BRDN0001146369)

   Plasmid

   #76912

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RIPK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RIPK2 (RIPK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TEK gRNA (BRDN0001146784)

   Plasmid

   #75722

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TEK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TEK (TEK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TEK gRNA (BRDN0001148973)

   Plasmid

   #75724

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TEK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TEK (TEK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. NRK gRNA (BRDN0001147558)

   Plasmid

   #76314

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NRK

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   NRK (NRK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TIE1 gRNA (BRDN0001147382)

   Plasmid

   #76686

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TIE1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TIE1 (TIE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5-AMLn-CAGEn-NES (Npu)

   Plasmid

   #241407

   Purpose

   Proximity-triggered Protein Trans-Splicing to generate the splice product AML1-ETO, Extended Figure 3 in Nature Chemical Biology 20.10 (2024): 1353-1360.

   Depositor

   Thomas Muir

   Article

   Lee et al bioRxiv [Preprint]. 2023 Jul 10:2023.07.

   Insert

   AMLn-CAGEn-NES (Npu) (RUNX1 Synthetic, Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Tnnt2 -Atp2a2-HA

   Plasmid

   #227804

   Purpose

   cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of mouse Atp2a2 and a HA tag

   Depositor

   Yuxuan Guo

   Article

   Chen et al Fundam Res. 2025 Jun 30. doi: https://do

   Insert

   Atp2a2-HA (Atp2a2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tnnt2

   Available since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-20XUAS-IVS-Syn21-Positron2-p10

   Plasmid

   #239082

   Purpose

   Gal4 activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Eric R. et al Janelia Figshare 2023

   Insert

   Positron2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R78K N81D D92N W178F

   Promoter

   hsp70

   Available since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-JFRC59-13XLexAop2-IVS-Syn21-Positron2-p10

   Plasmid

   #239081

   Purpose

   LexA activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Eric R. et al Janelia Figshare 2023

   Insert

   Positron2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R78K N81D D92N W178F

   Promoter

   hsp70

   Available since

   July 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-U6-sgRNA-BFP-Puro

    Plasmid

    #229014

    Purpose

    Perturb Seq lentiviral sgRNA vector

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

    Plasmid

    #203451

    Purpose

    Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Inserts

    U6-non targeting (NT) sgRNA
    Cas13e
    

    Use

    AAV

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Codon optimised using GenScript tool

    Promoter

    CMV and U6

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCA528 IST1 (322-366)

    Plasmid

    #193038

    Purpose

    Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 322-366

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-CAPN7-OSF

    Plasmid

    #193041

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528 CAPN7 L61D (1-165)

    Plasmid

    #193033

    Purpose

    Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    L61D, Residues 1-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-hU6-crCoV-Mutiplex_EF1a-BFP

    Plasmid

    #224788

    Purpose

    SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    crCoV20, crCoV21, crCoV24
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and hU6-2xTetO

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. DG SaCas9 GFP V3

    Plasmid

    #226961

    Purpose

    CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DG SaCas9 puro V3

    Plasmid

    #226960

    Purpose

    CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PDG458 eSp(1.1) V3

    Plasmid

    #226964

    Purpose

    CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHR-CD28-mTFP1

    Plasmid

    #223638

    Purpose

    Lentiviral expression of human CD28 with mTFP1 tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mTFP1

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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