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pRP-B-catenin-V5-LgBiT

Plasmid

#241816

Purpose

Expresses V5-tagged and LgBiT-tagged B-catenin for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

B-catenin

Tags

LgBiT and V5

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE8e–superNLS

Plasmid

#232429

Purpose

Expression plasmid for the ABE8e base editor with superNLS

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

ABE8e–superNLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgKEAP1-2

Plasmid

#186459

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FOXM1c

Plasmid

#68810

Purpose

Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

Depositor

Adam Karpf

Article

Barger et al Oncotarget. 2015 Jul 16.

Insert

FOXM1c (FOXM1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-Caspase-1-mNeonGreen

Plasmid

#166784

Purpose

FRET analysis (pro caspase-1 cleavage) in living cells

Depositor

Hao Wu

Article

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

Plasmid

#217704

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGR6 (ADGRG6 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-840

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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AcGFP-MSH6

Plasmid

#215123

Purpose

Expresses AcGFP-MSH6 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MSH6 (MSH6 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Src Y527E

Plasmid

#246428

Purpose

Expresses Src mutant Y527E (aka Y530E) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Tyrosine 530 to Glutamate

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-Src Y527A

Plasmid

#246429

Purpose

Expresses Src mutant Y527A (aka Y530A) in Mammalian cells

Depositor

Katharine White

Article

Van Dyck et al Sci Signal. 2025 Nov 11;18(912):eadt3018

Insert

Src (SRC Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Tyrosine 530 to Alanine

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEMAX-NLS-P2A_BLAST

Plasmid

#135355

Purpose

Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

ABEMax-2XNLS

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _Gata6

Plasmid

#73537

Purpose

Inducible lentiviral expression of Gata6

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgKEAP1-3

Plasmid

#186837

Purpose

knock out KEAP1 in mammalian cells

Depositor

Boyi Gan

Article

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pb-CAGGS-dCas9-VP64-blasto

Plasmid

#218556

Purpose

Encodes CAG promoter-driven dCas9-VP64 with a bipartite SV40 NLS between dCas9 and VP64, along with blasticidin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

dCas9

Use

CRISPR; Piggybac transposition

Tags

2xSV40NLS-VP64

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iAChSnFR

Plasmid

#137950

Purpose

Expresses iAChSnFR under hSynapsin promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Has Service

AAV1

Insert

iAChSnFR

Use

AAV

Expression

Mammalian

Promoter

hSynanpsin

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-53BP1 1-1972 WT

Plasmid

#53446

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway Cloning

Mutation

siRNA resistant and mutations A128V, T720A, A1347…

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE

Plasmid

#226381

Purpose

Expresses tau propagation ORF with V5 tag on WT 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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