19,654 results

1. pRK5-HA-SLC38A9.1

   Plasmid

   #71869

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pHAGE hSPC-eGFP-W

   Plasmid

   #36450

   Depositor

   Darrell Kotton

   Article

   Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

   Inserts

   human SP-C promoter
   eGFP (eGFP )
   

   Use

   Lentiviral

   Promoter

   N/A and human SP-C promoter

   Available Since

   May 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcdna3.1 hciap1

   Plasmid

   #8311

   Depositor

   Jon Ashwell

   Article

   Li et al Nature 2002 Mar 21;416(6878):345-7.

   Insert

   cIAP1 (BIRC2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-CMV-TetO2_3C-Twin-Strep

   Plasmid

   #113883

   Purpose

   Mammalian (inducible) protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CIBN-CAAX

   Plasmid

   #79574

   Purpose

   expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   CIBN (CIB1 Mustard Weed)
   

   Tags

   C-terminal CAAX-box

   Expression

   Mammalian

   Mutation

   K93A, R94A, K106A, K107A (please see depositor co…

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSC189

   Plasmid

   #32114

   Depositor

   Eric Rubin

   Article

   Chiang et al Gene. 2002 Aug 21;296(1-2):179-85.

   Type

   Empty backbone

   Use

   Transposon delivery vector

   Tags

   3x FLAG

   Available Since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PCR4-Shh::lacZ-H11

   Plasmid

   #139098

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

   Depositor

   Len Pennacchio

   Article

   Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Shh

   Available Since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

   Plasmid

   #89991

   Purpose

   Donor template for generation of SOX2-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

   Insert

   SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
   

   Use

   CRISPR

   Promoter

   none

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. OmpA3-PNGaseF-TEV-His6

   Plasmid

   #114274

   Purpose

   Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

   Depositor

   Martin Caffrey

   Insert

   Peptide: N-glycosidase F
   

   Tags

   OmpA3 leader sequence (for periplasmic localisati…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDsRed-Sensor

    Plasmid

    #22743

    Depositor

    Lynn Hudson

    Article

    Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

    Insert

    modified pCMV-DsRed-Express (Clontech)
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSCV PIG (Puro IRES GFP empty vector)

    Plasmid

    #21654

    Purpose

    Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA GFP10-ZIP-GFP11

    Plasmid

    #182243

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP-11
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3/Flag-METTL3

    Plasmid

    #53739

    Purpose

    For expression of METTL3 (methyltransferase-like 3) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 3 (METTL3 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-VSVM

    Plasmid

    #64086

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

    Depositor

    Ian Wickersham

    Insert

    VSV matrix protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid

    #68214

    Purpose

    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    P19 silencing suppressor
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBA560

    Plasmid

    #186236

    Purpose

    pTet-eLbuCas13a crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 puro Nodamura B2

    Plasmid

    #17228

    Depositor

    Christopher Sullivan

    Article

    Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

    Insert

    Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)
    

    Expression

    Mammalian

    Mutation

    2 silent point mutations

    Available Since

    June 22, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pmRFP-LC3

    Plasmid

    #21075

    Purpose

    Mammalian expression of rat LC3 fused to mRFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge