59,541 results

1. pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

   Plasmid

   #195548

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV 4xSTAR-mNeonGreen-NLS-blast

   Plasmid

   #136258

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mNeonGreen-NLS

   Expression

   Mammalian

   Available Since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. hFLT1

   Plasmid

   #83435

   Purpose

   human VEGFR1 with HA and FLAG tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

   Insert

   human VEGFR1 (FLT1 Human)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   HA-tag was inserted 30 AA downstream of the FLT1 …

   Promoter

   CMV

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. R777-E100 Hs.GRB2-nostop

   Plasmid

   #70384

   Purpose

   Gateway ORF clone of human GRB2 [NM_002086.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   GRB2 (GRB2 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. R777-E126 Hs.MAPK1-nostop

   Plasmid

   #70410

   Purpose

   Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-NPC1_STOP

   Plasmid

   #161461

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   NPC1 (NPC1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. HMGCS1

   Plasmid

   #38844

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HMGCS1 (HMGCS1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. A*11:01:01:01

   Plasmid

   #203276

   Purpose

   Overexpress HLA allele

   Depositor

   Derin Keskin, Catherine Wu

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*11:01:01:01 (HLA-A Human)
   

   Expression

   Mammalian

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo beta catenin delta45

   Plasmid

   #16520

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   beta catenin (CTNNB1 Human)
   

   Expression

   Mammalian

   Mutation

   Delta 45 (HCT116 mutation)

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

    Plasmid

    #172627

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGloSensor -40F cGMP Vector

    Plasmid

    #236812

    Purpose

    Express -40F cGMP in biosensor assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    -40F
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP

    Plasmid

    #181980

    Purpose

    Human RUNX1C gene overexpression

    Depositor

    Jan-Henning Klusmann

    Insert

    RUNX1C (human) (RUNX1 Human)
    

    Use

    Lentiviral

    Tags

    HA (Human influenza hemagglutinin)

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP1B

    Plasmid

    #73241

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FKBP1B (FKBP1B Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

14. LRRK2_Halo_N_allele

    Plasmid

    #178142

    Purpose

    Donor vector for endogenous tagging of human LRRK2 at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (LRRK2 Human)
    

    Use

    Donor vector

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA Flag Yap1

    Plasmid

    #18881

    Depositor

    Yosef Shaul

    Article

    Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

    Insert

    Yap1 (YAP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-myc3-ROC1

    Plasmid

    #20717

    Depositor

    Yue Xiong

    Article

    He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Tags

    3xMyc

    Expression

    Mammalian

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFGAMS-mCherry

    Plasmid

    #166024

    Purpose

    Expresses human PFAS-mCherry fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

    Insert

    PFAS-mCherry (PFAS Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sec61 beta pAcGFP-C1 (802)

    Plasmid

    #62008

    Purpose

    mammalian expression of GFP-Sec61 beta

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. mCherry-Vimentin-N-18

    Plasmid

    #55158

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Vimentin (VIM Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-p73α-pcDNA3

    Plasmid

    #22102

    Depositor

    William Kaelin

    Article

    Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

    Insert

    tumor protein p73 (TP73 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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