We narrowed to 13,726 results for: cas9

1. pCitro-15a

   Plasmid

   #202033

   Purpose

   p15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to Citrobacter

   Depositor

   David Edgell

   Article

   Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

   Insert

   conjugative system from a Citrobacter spp.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-TC9

   Plasmid

   #202088

   Purpose

   entry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-dest

   Depositor

   David Edgell

   Article

   Hamilton et al ACS Synth Biol. 2023 Dec 15;12(12):3578-

   Insert

   TevCas9 + sgRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.103

   Plasmid

   #184988

   Purpose

   Express Cas9-P2A-Eco1RT

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-P2A-Eco1RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCL.102

   Plasmid

   #184987

   Purpose

   Express -Eco1 RT-P2A-Cas9

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-P2A-Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.005

   Plasmid

   #184971

   Purpose

   Express Cas9 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Cas9
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   Promoter

   Gal1-10

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-T Easy-AtpU6-29

   Plasmid

   #160219

   Purpose

   AtpU6-29 Golden Gate level 0 piece to express gRNAs

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   pAtU6-29 promoter
   

   Use

   Golden gate level 0 piece to express grnas

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOC4 Grin1-smFP-V5 KI

   Plasmid

   #183439

   Purpose

   FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and smFP V5 donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMiniT:myc-GluA2 TKIT donor

   Plasmid

   #169423

   Purpose

   Donor DNA for TKIT knockin of Myc to GluA2 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Myc (Myc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Gal4-RNF169UBD

   Plasmid

   #119013

   Purpose

   Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sg2

    Plasmid

    #113967

    Purpose

    Single short guide RNA targeting TACCACATTTGTAGAGGTT

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. sg3

    Plasmid

    #113968

    Purpose

    Single short guide RNA targeting CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sg2+sg3

    Plasmid

    #113970

    Purpose

    Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg2+sg3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-hsg(NT:NT)

    Plasmid

    #138260

    Purpose

    Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    Nontargeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB1C3-FeGFP

    Plasmid

    #138261

    Purpose

    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJM108

    Plasmid

    #134160

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    KMLL, E. coli codon optimized, CRISPR/Cas9 harden…

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM107

    Plasmid

    #134159

    Purpose

    Retroviral plasmid expressing GFP - Nde1

    Depositor

    Iain Cheeseman

    Article

    Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

    Insert

    Nde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SSSC, E. coli codon optimized, CRISPR/Cas9 harden…

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. BII-C3H

    Plasmid

    #133386

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. BII-B3HTK

    Plasmid

    #133389

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCF736

    Plasmid

    #121893

    Purpose

    EF1a-dTEV-protease-GFP

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Inserts

    TEV protease
    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNOC-CRISPR-compact

    Plasmid

    #99369

    Purpose

    Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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