Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,334 results for: gateway

Showing: 11241 - 11280 of 18334 results

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pAG415Gal_FL Gcn4

Plasmid

#232955

Purpose

Galactose iduced expression of full-length Gcn4 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120AHA

Plasmid

#232992

Purpose

Galactose iduced expression of Gcn4 W120AHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDHA

Plasmid

#232987

Purpose

Galactose iduced expression of Gcn4 DBDAHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 L123A

Plasmid

#232969

Purpose

Galactose iduced expression of Gcn4 L123A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap2

Plasmid

#232965

Purpose

Galactose iduced expression of Gcn4 k0.19 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.19

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3

Plasmid

#232960

Purpose

Galactose iduced expression of Gcn4 k0.47 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R2

Plasmid

#232970

Purpose

Galactose iduced expression of Gcn4 R+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R+

Tags

TEV cleavage site

Expression

Yeast

Mutation

K118R, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E+

Plasmid

#232963

Purpose

Galactose iduced expression of Gcn4 E+in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 W120A

Plasmid

#232962

Purpose

Galactose iduced expression of Gcn4 W120A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3

Plasmid

#232973

Purpose

Galactose iduced expression of Gcn4 R++ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103R, D133R, K143R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3GFP

Plasmid

#233010

Purpose

Galactose iduced expression of Gcn4 k0.47GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBD

Plasmid

#232985

Purpose

Galactose iduced expression of Gcn4 DBD in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F124I

Plasmid

#232964

Purpose

Galactose iduced expression of Gcn4 F124I in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Swap3HA

Plasmid

#232998

Purpose

Galactose iduced expression of Gcn4 k0.47HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 k0.47

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 F108A+

Plasmid

#232974

Purpose

Galactose iduced expression of Gcn4 F108A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A

Tags

TEV cleavage site

Expression

Yeast

Mutation

F108A, T121L, A141P

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_DBDGFP

Plasmid

#232999

Purpose

Galactose iduced expression of Gcn4 DBDGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

DBD

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 WT 30mer

Plasmid

#231888

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 30mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_DBD

Plasmid

#231886

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 DBD

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 DBD

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 StoL

Plasmid

#231882

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 StoL

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104L, S117L, S136L, S144L

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 Swap1

Plasmid

#231879

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap1

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap1

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 R3

Plasmid

#231874

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103R, D133R, K143R

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 L113A+

Plasmid

#231873

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L113A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 P+

Plasmid

#231872

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 P+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 P+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S122P, T131F, A141P, E143D

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 E2K2

Plasmid

#231868

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E2K2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E2K2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104K, S117E, S136E, S144K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 K5

Plasmid

#231866

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 K5

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 E3

Plasmid

#231863

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E3

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D105E, D118E, D139E

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 W120A

Plasmid

#231862

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 W120A

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

W120A

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR4 HopU1

Plasmid

#228395

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

HopU1

Use

Gateway-compatible entry vector

Mutation

Alanine inserted after start Methionine due to Nc…

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#233545

Purpose

A CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Lentiviral

Tags

V5

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-CMV-EGFPCAAX

Plasmid

#232140

Purpose

Expresses EGFP with a CAAX motif under control of a CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

EGFPCAAX

Use

Zebrafish expression

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ265_BasA3G

Plasmid

#225145

Purpose

Gateway entry clone (attL1 & attR5) BasA3G-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Inserts

BasA3G-zCas9(D10A)-UGI
-

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ265_EtA3C

Plasmid

#225146

Purpose

Gateway entry clone (attL1 & attR5) EtA3C-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

EtA3C-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ265_DlA3G

Plasmid

#225148

Purpose

Gateway entry clone (attL1 & attR5) DlA3G-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

DlA3G-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ265_LoA3GX1

Plasmid

#225149

Purpose

Gateway entry clone (attL1 & attR5) LoA3GX1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

LoA3GX1-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 Aro3+

Plasmid

#231884

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro3+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 Aro2+

Plasmid

#231883

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F108A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 R2

Plasmid

#231870

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

K118R, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 L123A+

Plasmid

#231869

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L123A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123A

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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