159,530 results

1. pDonor_Tn[himar1C9]

   Plasmid

   #213906

   Purpose

   Mariner transposon with ATc-inducible himar1C9 transposase

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBABE-SSeCKs

   Plasmid

   #106908

   Purpose

   full-length murine SSeCKs/AKAP12/Gravin in retroviral vector

   Depositor

   Heidi Hehnly

   Article

   Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

   Insert

   SSeCKs (Akap12 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   gag

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mPA-GFP-Tubulin-C-18

   Plasmid

   #57160

   Purpose

   Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. hATMS1981A

   Plasmid

   #32300

   Depositor

   Michael Kastan

   Article

   Bakkenist et al Nature. 2003 Jan 30;421(6922):499-506.

   Insert

   ATM (ATM Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   S1981A

   Promoter

   Available since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1321 HPV-16 E6/E7

   Plasmid

   #8641

   Depositor

   Peter Howley

   Article

   Münger et al J Virol. 1989 Oct . 63(10):4417-21.

   Insert

   HPV-16 E6/E7 (E7, E6 Human papillomavirus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCiES

   Plasmid

   #60010

   Purpose

   Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.

   Depositor

   David Russell

   Article

   Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

   Insert

   Env (foamy virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

   Plasmid

   #210525

   Purpose

   AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mtagBFP-KASH
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLJM1 Flag Raptor

   Plasmid

   #26633

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-mPCSK3

   Plasmid

   #122674

   Purpose

   Expresses mouse PCSK3 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP

    Plasmid

    #165830

    Purpose

    EGFP green fluorescent protein CDS

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCerulean N1

    Plasmid

    #27795

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Insert

    mCerulean N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLX1.8CMVMitoTurboRFP

    Plasmid

    #28131

    Depositor

    Geoffrey Owens

    Article

    Chen et al PLoS One. 2008 . 3(7):e2804.

    Insert

    MitoTurboRFP
    

    Use

    Lentiviral

    Tags

    COX VIII signal sequence

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid

    #127390

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHCD1C

    Plasmid

    #102954

    Purpose

    gene cloning of human CD1C

    Depositor

    Lucia Mori, Gennaro De Libero

    Article

    Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

    Insert

    CD1C cDNA (CD1C Human)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. htrpC6-YFP

    Plasmid

    #21084

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    TrpC6 (TRPC6 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

    Plasmid

    #211471

    Purpose

    lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF-1a

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-Nesprin-1αKASH

    Plasmid

    #187015

    Purpose

    Disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

    Insert

    Nesprin-1alphaKASH
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HSPA9A

    Plasmid

    #42469

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSPA9A (HSPA9 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Flag-FOXG1

    Plasmid

    #153124

    Purpose

    Expression of Flag tagged human FOXG1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXG1 (FOXG1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETM6-vioABECD

    Plasmid

    #66535

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCiGSΔΨ

    Plasmid

    #108312

    Purpose

    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

    Insert

    gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCiGSΔΨ is a newer version of the pCiGS Gag expre…

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TFORF0884

    Plasmid

    #142702

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSG5 Large T

    Plasmid

    #9053

    Depositor

    William Hahn

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TetO-FUW-NfiA

    Plasmid

    #64901

    Purpose

    Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    Nfia (Nfia Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. medSIIN

    Plasmid

    #185664

    Purpose

    shApc-expressing lentiviruses with varius levels of expression of SIINFEKL

    Depositor

    Tyler Jacks

    Article

    Westcott et al Nat Cancer. 2021 Oct;2(10):1071-1085. do

    Inserts

    mScarlet
    miR-30a
    shApc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mPA-GFP-Actin-C-18

    Plasmid

    #57121

    Purpose

    Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. MYR-FR-C2D-EGFR

    Plasmid

    #179262

    Purpose

    Light-induced clustering of EGFR cytosolic domains

    Depositor

    Jared Toettcher

    Article

    Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Cry2 PHR, FUS, FusionRed, and Myristoylation sequ…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEZYmyc-His

    Plasmid

    #18701

    Purpose

    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX_TRC311 ETV4-S

    Plasmid

    #74983

    Purpose

    Constitutive ETV4 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 )
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mEos3.2-Vimentin-7

    Plasmid

    #57485

    Purpose

    Localization: Intermediate Filaments, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

32. pV238-vPE

    Plasmid

    #225258

    Purpose

    Mammalian expression of vPE

    Depositor

    Vikash Chauhan, Robert Langer, Phil Sharp

    Article

    Chauhan et al bioRxiv 2024.08.02.606370

    Insert

    vPE
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    R221K K848A H982A N1317R

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. N-BLa 1.2

    Plasmid

    #88999

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.2 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. C-BLa 1.2

    Plasmid

    #89000

    Purpose

    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    C-BLa 1.2 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKIKOarsBCm

    Plasmid

    #46763

    Purpose

    E.coli genomic integration at arsB

    Depositor

    Claudia Vickers, Lars Nielsen

    Article

    Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

    Type

    Empty backbone

    Use

    E.coli genomic integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. reporter-gT1

    Plasmid

    #47320

    Purpose

    TF reporter for gRNA-AAVS1_T1 (RNA-guided dTomato expression)

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    minCMV-dTomato_pA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pVITRO1-dV-IgG1/κ

    Plasmid

    #52213

    Purpose

    Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin kappa constant region
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deleted Variable region

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GST-TAX1BP1

    Plasmid

    #171423

    Purpose

    For the purifcation of TAX1BP1 protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTBL3478 PB-PEmax-mTagBFP2

    Plasmid

    #226664

    Purpose

    PiggyBac cargo vector encoding PEmax and mTagBFP2

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Inserts

    PEmax
    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and UBC

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge