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pJKR-O-acuR

Plasmid

#62569

Purpose

Acrylate biosensor. Medium copy plasmid that expresses CFP proportional to acrylate concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

CFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBbE7c-RFP

Plasmid

#35314

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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mCD8-GFP in pBS

Plasmid

#17745

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

mouse CD8-GFP (Cd8a Mouse)

Tags

GFP

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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P1-M-P2

Plasmid

#87091

Purpose

This is the P1-M-P2 portion of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pBbE6c-RFP

Plasmid

#35287

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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p153 pPr-cre

Plasmid

#8403

Purpose

Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin driven Cre

Use

Cre/Lox

Expression

Mammalian

Promoter

probasin promoter

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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optGluCl alpha EYFP

Plasmid

#15104

Depositor

Henry Lester

Article

Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

Insert

GluCl alpha (glc-1 Nematode)

Tags

EYFP

Expression

Mammalian

Mutation

Codon optimized cDNA

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only

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pH6HTC_MANF-Halo

Plasmid

#175341

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

MANF (MANF Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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M-SP-sgRNA

Plasmid

#48671

Purpose

Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pGC185

Plasmid

#19624

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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PKC delta dNPS

Plasmid

#16387

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 15…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
LIIbeta F 1-2 RNAi

Plasmid

#54403

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D GUS

Plasmid

#104517

Purpose

LI Gene of Interest module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

UidA gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBbB5a-GFP

Plasmid

#35352

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO.1-MKL1/2 shRNA

Plasmid

#27161

Purpose

Lentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2.

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

shRNA MKL1+2

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt2b

Plasmid

#42275

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt2b (Wnt2b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pG12-Rluc8

Plasmid

#170272

Purpose

Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

RLuc8

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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