We narrowed to 65,520 results for: nin
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Plasmid#71484PurposeExpresses palindromic sgRNA for use in self-cloning CRISPRDepositorInsertsgPal7
PromoterU6Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL056 pDESTattB_exorh:EGFP
Plasmid#213510PurposeattB-containing pDEST plasmid, for use in Multisite Gateway reactionDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT120b
Plasmid#122604PurposeBE PACE selection, express gIII, luxAB and degron-GTCCA guide RNADepositorInsertgIII, luxAB, guide RNA
UseSynthetic BiologyAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-BSD
Plasmid#174819PurposeMammalian expression of SpCas9 prime editor 2 with P2A-BSD markerDepositorInsertPE2-P2A-BSD
TagsP2A-BSD and SV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
Plasmid#154773Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125A in G1PylS, hybrid PylT with A41AA and C55A …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB6a
Plasmid#19502DepositorAvailable SinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDY1314_RADARS_Luciferase
Plasmid#193191PurposeExpress Gaussia Luciferase conditional upon RNA transcriptDepositorInsertRADARS Luciferase
ExpressionMammalianAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBH533_MBP-Cas14b2 expression
Plasmid#112506PurposeExpresses 10xHis-MBP fused to Cas14b2 under t7 promoterDepositorInsertCas14b2
UseCRISPRTagsMBPExpressionBacterialPromoterT7Available SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-ISceID44A
Plasmid#59424PurposeExpresses a catalytically inactive form of the endonuclease ISceIDepositorInsertendonuclease ISceI mutant (I-SceI )
TagsHA tagExpressionMammalianMutationchanged aspartate 44 to alanine (D44A)Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE DN Cullin2
Plasmid#41912DepositorInsertCUL2 (CUL2 Human)
UseLentiviralAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEF-ENTR C (709-1)
Plasmid#17429PurposeGateway entry vector with EF1-a promoter, V5-His C-term, frame CDepositorTypeEmpty backboneUseGateway CloningExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET30-RT1306
Plasmid#131521PurposeExpresses engineered RT-1306 with His-tagDepositorInsertRT-1306
TagsHis-tagExpressionBacterialPromoterT7Available SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-Lambda2
Plasmid#104184PurposeLentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-sgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETconNK
Plasmid#81169PurposepETconNK Empty BackboneDepositorTypeEmpty backboneTagsc-mycExpressionBacterial and YeastPromoterGAL1Available SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGolden-AAV
Plasmid#51424PurposeContain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reactionDepositorInsertlacZ
UseAAVPromoterlacZAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ353 - MCS(ttTi4391, I)
Plasmid#34869DepositorInsertttTi4391 targeting
UseWorm targetingAvailable SinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
Double UP mScarlet to mNeon
Plasmid#125139PurposeNatively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.DepositorTypeEmpty backboneExpressionMammalianMutationRemoved Not1 site in mScarletAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER-B
Plasmid#209870PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP B
TagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only