We narrowed to 6,260 results for: pcDNA 3.1
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Rlucrarr3-Flash3
Plasmid#74131PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash2
Plasmid#74130PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.DepositorInsertArrestin3
TagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…Available SinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald(luminal)-canxTM
Plasmid#186961PurposeExpresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.DepositorInsertCANX (CANX Human)
TagsmEmeraldExpressionMammalianMutationOnly express calnexin (482-end) with ER signal pe…PromoterCMV PromoterAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
VN-Sph1
Plasmid#89755Purposeexpresses human Synphilin fused to the N-terminal part of VenusDepositorAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
CoV2-N-B.1.1.7
Plasmid#177958PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.DepositorInsertSARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
ExpressionMammalianMutationD3L,R203K,G204R,S235FAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2
Plasmid#66948PurposeMammalian expression of rat ErbB2 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
Tags6xHis, V5 Tag, and YFPExpressionMammalianMutation(See depositor comment below on mutations A24T an…PromoterCMVAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
nuc-GafD-mTurboID-HA
Plasmid#184641PurposeExpresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome taggingDepositorInsertnuc-GlycoID-HA
Tags3xHA tag and Nuclear localization sequenceExpressionMammalianMutationGafD -> expressed 22-178 ; BirA* -> express…PromoterCMVAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD
Plasmid#159999PurposeExpresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
ExpressionMammalianMutationnoneAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mito-mTurq2-Apollo-NADP+
Plasmid#193303PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the mitochondriaDepositorInsertG6PD (G6PD Human)
TagsmTurqoise2 and mitochondrial targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available SinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD406
Plasmid#163111PurposeExpression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mNeonGreen_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD399
Plasmid#163106PurposeExpression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_mEGFP_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED22
Plasmid#15423DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD10
Plasmid#195848PurposeExpresses human FZD10 with an N-terminal HiBiTDepositorAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hIes2
Plasmid#15355DepositorAvailable SinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
NSP4-WT-Flag
Plasmid#210339PurposeExpresses SARS-CoV-2 NSP4 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
TagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCD4-TagRFP-T (RHPMP151)
Plasmid#119238PurposeExpresses CD4 fused to TagRFP-TDepositorAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PH Akt-Venus
Plasmid#85223PurposeVenus tagged PH domain of Akt for use as a PIP3 sensorDepositorAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-dCT
Plasmid#126422PurposePlasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertCalcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
TagsFLAGExpressionMammalianAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only
