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We narrowed to 10,745 results for: AGA

Showing: 8461 - 8490 of 10745 results

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pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

Plasmid

#210741

Purpose

Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp Cas9
Merry sgRNA GFP Target 1

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

N-terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

Plasmid

#210742

Purpose

Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

SaSp Cas9
Merry sgRNA GFP Target 2

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Mutation

N-terminal Sa Cas9, C-terminal Sp Cas9

Promoter

H1 and eCMV

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-dErbB4

Plasmid

#197357

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-bleo-ErbB4

Plasmid

#197353

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-dErbB2

Plasmid

#197355

Purpose

A knock-out vector for dog ErbB2.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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p5’UTR-ORF1

Plasmid

#190566

Purpose

Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).

Depositor

Anthony Furano

Article

Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1

Use

For dna propagation only

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1067L

Plasmid

#200253

Purpose

pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

6 gRNAs targeting Doublesex, Intersex, bTublin

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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tet pLKO.1-shNUP37 v2 puro

Plasmid

#192344

Purpose

Lentiviral expression vector for an inducible shNUP37 v2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

shNUP37 v2 (NUP37 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

Promoter

EF1a/hU6

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgSNRPA guide 1

Plasmid

#193598

Purpose

SNRPA knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgSNRPA guide 1 (SNRPA Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003_sgTFAP4 guide 2

Plasmid

#193587

Purpose

TFAP4 knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgTFAP4 guide 2 (TFAP4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A-Blimp1-L2-#1

Plasmid

#171505

Purpose

deletion of a genomic locus in Blimp1(Prdm1) gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Blimp1 (Prdm1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Prdm14-ex1-#1

Plasmid

#171507

Purpose

deletion of a genomic locus in Prdm14 gene

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

Prdm14 (Prdm14 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBXPHM3-NB-D6

Plasmid

#167989

Purpose

expression of a nanobody against rat PepT2 as a n terminal MBP fusion

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

NanobodyD6

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_3

Plasmid

#190705

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 3

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DPB

Plasmid

#164989

Purpose

Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPB1*01:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DQB

Plasmid

#164991

Purpose

Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DQB1*05:01:01

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_DPA

Plasmid

#164988

Purpose

Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA-DPA1*02:01:08

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.1 (Hist1h3g) sgRNA

Plasmid

#186930

Purpose

Hist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hist1h3g sgRNA (H3c8 Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAP01

Plasmid

#186260

Purpose

sfGFP under light light responsive PEL222 promoter

Depositor

Yongku Cho

Article

Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

Plasmid

#73543

Purpose

Expression of sgRNA targeting LINE-1 and TagBFP

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

LINE-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CYP19A1

Plasmid

#183280

Purpose

All-in-One CRISPRko system with a guide RNA that targets CYP19A1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CYP19A1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DHFR

Plasmid

#183281

Purpose

All-in-One CRISPRko system with a guide RNA that targets DHFR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DHFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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1056E

Plasmid

#183133

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[dsx]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MET

Plasmid

#183310

Purpose

All-in-One CRISPRko system with a guide RNA that targets MET gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MET

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC1

Plasmid

#183297

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CSF3R

Plasmid

#183278

Purpose

All-in-One CRISPRko system with a guide RNA that targets CSF3R gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CSF3R

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pOC4 Kcnn2-smFP-myc KI

Plasmid

#183438

Purpose

FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP myc donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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