We narrowed to 25,556 results for: lars

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pEM3 = ncs-1p::nCre

Plasmid

#24034

Depositor

Cori Bargmann

Article

Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Insert

ncs-1 (ncs-1 Nematode)

Use

Cre/Lox

Tags

nCre

Expression

Worm

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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sc AAV miR26a eGFP

Plasmid

#21894

Depositor

Joshua Mendell

Article

Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

Insert

EF1 alpha -miR26a GFP p(A)

Use

AAV; Sc

Tags

GFP

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pHCMV_MERS-CoV_S

Plasmid

#207293

Purpose

Mammalian expression of MERS coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MERS-CoV_S (S MERS coronavirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLux76-LasI

Plasmid

#73444

Purpose

HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL

Depositor

Jim Haseloff

Article

Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

Insert

LasI

Use

Synthetic Biology

Promoter

pLux76

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJUMP24-1A(sfGFP)

Plasmid

#126971

Purpose

Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE G1

Plasmid

#221978

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE C2

Plasmid

#221979

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE T1

Plasmid

#221980

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE T2

Plasmid

#221981

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a - NR

Plasmid

#89625

Purpose

Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Nitrate reductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAd5-B6/7deltaE3

Plasmid

#175748

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 25043-35938

Use

Adenoviral and Synthetic Biology

Mutation

Adenovirus sequences 27859-30803 replaced with Ba…

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MTK2_005

Plasmid

#123700

Purpose

Encodes pCAG as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCAG

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pKeratin-LSSmOrange

Plasmid

#37138

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human cytokeratin 18

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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Eps8-mCherry-C1

Plasmid

#29779

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Eps8 (Eps8 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only
pET51b(+)-SNAP-CLIP

Plasmid

#127180

Purpose

Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-CLIP

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt1 I367T IRES mRuby3

Plasmid

#170642

Purpose

Expression of syt1 in cultured neurons by lentiviral transduction

Depositor

Edwin Chapman

Article

Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

Insert

SYT1 (Syt1 Rat)

Use

Lentiviral

Mutation

I367T

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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APPL1-pmCherryC1

Plasmid

#27683

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

APPL1 (Appl1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pWS5398-CRISPRai-HygR

Plasmid

#182837

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMM310-Myc

Plasmid

#80053

Purpose

Expression of a Myc tagged version of the BlaM-Vpr fusion protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-BlaM-Vpr

Tags

Myc

Expression

Mammalian

Mutation

N-terminal Myc tag added to construct pMM310

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_PCMT1_PCMT

Plasmid

#109932

Purpose

Protein expression and purification of PCMT1_PCMT

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PCMT1_PCMT

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pMOS018: ratiometric pHluorin (mitchondrial)

Plasmid

#163053

Purpose

ratiometric pHluorin (mitchondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_PABPC1_PABP

Plasmid

#109931

Purpose

Protein expression and purification of PABPC1_PABP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PABPC1_PABP

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-FAF1

Plasmid

#107986

Purpose

expression of FAF1

Depositor

Georg Weber

Article

Weber et al Sarcoma. 2015;2015:839182. doi: 10.1155/

Insert

FAF1 (FAF1 Human)

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Myc-hZO-1-HA

Plasmid

#236528

Purpose

For expression of ZO-1 (human) in mammalian cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Tags

HA and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMOS018E: Entry vector for: ratiometric pHluorin (mitchondrial)

Plasmid

#163070

Purpose

Entry vector for: ratiometric pHluorin (mitchondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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B2088_BTB-IDR-FRB

Plasmid

#215101

Purpose

Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-mCherry-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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GEV 2

Plasmid

#12616

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with IgT antigen as insert (SV40gp6 SV40)

Tags

G protein and His

Expression

Bacterial

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pOGG005

Plasmid

#113980

Purpose

Level 1 high copy number vector for E. coli expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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