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pKlab223-pcDNA3-BRCA1(1314-end)-R1699Q-V5-3xFLAG

Plasmid

#249020

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the R1699Q mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

R1699Q

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab228-pcDNA3-BRCA1(1314-end)-S1715R-V5-3xFLAG

Plasmid

#249021

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the S1715R mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

S1715R

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab189-pcDNA3-BRCA1(1314-end)-A1708E-V5-3xFLAG

Plasmid

#249022

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the A1708E mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708E

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab521-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-Y1845F-STOP

Plasmid

#249009

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the Y1845F mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845F

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab522-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-Y1845D-STOP

Plasmid

#249010

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the Y1845D mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845D

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab524-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-G1788V-STOP

Plasmid

#249011

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the G1788V mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

G1788V

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab518-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-A1708E-STOP

Plasmid

#249012

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the A1708E mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708E

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab979-pcDNA3-BRCA1(1314-end)-A1708V-V5-3xFLAG

Plasmid

#249014

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the A1708V mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708V

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-ITGB1-V1

Plasmid

#215454

Purpose

Expression vector with integrin beta1 tagged with the BiFC V1 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CMV

Available Since

Nov. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB-CAG-shLRRK2-mCherry-CAAX

Plasmid

#227686

Purpose

Membrane tethered mCherry and shRNA targeting LRRK2

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

LRRK2 shRNA (Lrrk2 Mouse)

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shAtg7-mCherry-CAAX

Plasmid

#227687

Purpose

Membrane tethered mCherry and shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Atg7 shRNA (Atg7 Mouse)

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D-Ezrin T567A-BioID2-HA

Plasmid

#227683

Purpose

To over express Phospho-dead Ezrin under gfaABC1D promoter in astrocytes

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Ezrin (Ezr Mouse)

Tags

BioID2-HA

Expression

Mammalian

Mutation

T567A

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-HaloDAmut

Plasmid

#234409

Purpose

Expresses the chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut in neurons

Depositor

Yulong Li

Article

Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

Insert

GPCR activation based chemigenetic dopamine (DA) control sensor GRAB_HaloDAmut

Use

AAV

Promoter

hSyn

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pNK091

Plasmid

#219769

Purpose

MoClo-compatible Level P vector carrying fungal bioluminescence system lacking luciferase gene

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA-ATPT | p35S-nnCPH-ocsT | pFMV-nnH3H-nosT

Use

Synthetic Biology

Expression

Plant

Promoter

pNos-KanR-ocsT | p35S-nnHispS-ocsT | pCmYLCV-npgA…

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-HHEX

Plasmid

#222928

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of HHEX

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

HHEX (HHEX Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF883

Plasmid

#101608

Purpose

Donor Vector containing ZNF883 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF883 (ZNF883 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF234

Plasmid

#101611

Purpose

Donor Vector containing ZNF234 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF234 (ZNF234 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF233

Plasmid

#101558

Purpose

Donor Vector containing ZNF233 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF233 (ZNF233 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF516

Plasmid

#101566

Purpose

Donor Vector containing ZNF516 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF516 (ZNF516 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF579_isoform2

Plasmid

#101569

Purpose

Donor Vector containing ZNF579 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF579 (ZNF579 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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