We narrowed to 9,384 results for: Coli

1. pEPQD0KN0245

   Plasmid

   #162284

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEPQDKN0729

   Plasmid

   #162316

   Purpose

   TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   T7prom-RBS-NET-TEVprotease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSF-KpAGO207-1251

   Plasmid

   #117854

   Purpose

   Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

   Depositor

   Kotaro Nakanishi, Dinshaw Patel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   Argonaute
   

   Use

   Tags

   6xHis-SUMO encoded in vector

   Expression

   Bacterial

   Mutation

   missing first 206 amino acid residues

   Promoter

   T7

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-SznE

   Plasmid

   #139282

   Purpose

   expresses SznE in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   sznE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMOD4 galK-GT

   Plasmid

   #19183

   Depositor

   Al Fisher

   Article

   Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

   Insert

   galK (galK E.coli)
   

   Use

   Recombineering

   Tags

   50 nucleotides from N-term TAP, C-term TAP, and F…

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-PAN

   Plasmid

   #200971

   Purpose

   Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Insert

   PAN
   

   Use

   Tags

   6xHis and 6xHis-thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBbS5c-AcrB/AcrD/AcrFa, ABO_0964

   Plasmid

   #86206

   Purpose

   Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

   Depositor

   Mary Dunlop

   Article

   Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

   Insert

   Efflux pump
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-hdNadV

   Plasmid

   #83362

   Purpose

   Expresses nadV from Haemophilus ducreyi in Escherichia coli

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

   Insert

   nadV
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pND004

   Plasmid

   #200950

   Purpose

   MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

   Depositor

   Timothy Whitehead

   Article

   Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEDL3

    Plasmid

    #25092

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    Edge detector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAW-SpyCsn2

    Plasmid

    #113251

    Purpose

    E. coli expression vector for S. pyogenes Csn2-MBP-His

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Csn2
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSETb-pHlash

    Plasmid

    #61550

    Purpose

    Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification

    Depositor

    Carl Johnson

    Article

    Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

    Insert

    pHlash
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAW-SpyCas1

    Plasmid

    #113248

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas1
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAW-SpyCas2

    Plasmid

    #113249

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas2

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas2
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRD324

    Plasmid

    #198045

    Purpose

    Expression of HMfB D14A K30A E34A in E. coli

    Depositor

    Remus Dame

    Article

    Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

    Insert

    HMfB D14A K30A E34A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pet11a-ABLE

    Plasmid

    #158627

    Purpose

    Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

    Depositor

    William DeGrado

    Article

    Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

    Insert

    Apixaban-Binding Helical Bundle
    

    Use

    Tags

    6x His-tag and TEV cleavage tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcrRNA.ind

    Plasmid

    #61284

    Purpose

    Arabinose-inducible repeat-only E. coli CRISPR array

    Depositor

    Chase Beisel

    Article

    Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT105

    Plasmid

    #25089

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    Edge detector delta cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMAL-c5X-ELP[V5A2G3-60]

    Plasmid

    #67009

    Purpose

    Expresses malE-ELP[V5A2G3-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[V5A2G3-60]
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDW114

    Plasmid

    #70061

    Purpose

    artificial transcription template "wild-type"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only