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pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR

Plasmid

#195853

Purpose

Expresses inhibitory opsin gtACR2 and GFP in turning associated neurons

Depositor

Andrew Leifer

Article

Kumar et al PLoS Biol. 2023 Sep 21;21(9):e3002280. d

Insert

Guillardia theta anion-conducting channelrhodopsin-2 (GtACR2)

Expression

Worm

Promoter

lim-4

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B no-stop

Plasmid

#123205

Purpose

Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway Cloning

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A no-stop

Plasmid

#123204

Purpose

Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway Cloning

Mutation

Stop codon removed

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg1

Plasmid

#87189

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 1

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #1 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP1-His

Plasmid

#67869

Purpose

bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP1 (PRORP1 Mustard Weed)

Tags

His

Expression

Bacterial

Mutation

Residues 36-572 (see comments)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Rab8

Plasmid

#31735

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab8 (Rab8 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
CJ32 (let-7 minigene)

Plasmid

#17769

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-let-7 minigene (let-7 Fly)

Use

Drosophila p-element vector

Available Since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
CJ19 (miR-1006 minigene)

Plasmid

#17760

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1006 minigene

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pCK-Drosha(UniProt)-FLAG-mEGFP

Plasmid

#164691

Purpose

Express Drosha-FLAG-mEGFP in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

Drosha (DROSHA Human)

Tags

FLAG and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3C G126

Plasmid

#123079

Purpose

Gateway entry clone encoding human MAP1LC3C G126

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Use

Gateway Cloning

Mutation

Deleted amino acids 127-147. Stop codon after G12…

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pIMVAd39T

Plasmid

#98303

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein coiled-coil 2

Plasmid

#73522

Purpose

Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 2 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein coiled-coil 1

Plasmid

#73521

Purpose

Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 1 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP G116A

Plasmid

#123098

Purpose

Expresses 3xFLAG-GABARAP G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1-EGFP

Plasmid

#112857

Purpose

plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pCIS-krPAM2

Plasmid

#25457

Depositor

Richard Mains

Article

Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

Insert

PAM2 (Pam Rat)

Expression

Mammalian

Mutation

Exon A deleted (exon A extends from nt 1475 to n…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV A71 3C protease

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCF806-shABC1

Plasmid

#186713

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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