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BlatCas9

Plasmid

#163792

Purpose

The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN

Plasmid

#32789

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-146a

Plasmid

#64234

Purpose

Expresses miR-146a in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR146a (MIR146A Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-C

Plasmid

#183160

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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ninaE-norpA

Plasmid

#26854

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

no receptor potential A (norpA Fly)

Expression

Insect

Mutation

V963A

Available Since

April 5, 2011

Availability

Academic Institutions and Nonprofits only
pUC19/eCFP-C

Plasmid

#183156

Purpose

Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2m

Plasmid

#18713

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

R577L, K578M

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-CaM-V5-sTb(N)

Plasmid

#219779

Purpose

sTb(N) half of CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CaM-V5-sTb(N)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP670-2

Plasmid

#197201

Purpose

Expresses the protein of miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_MERS-CoV_S

Plasmid

#207293

Purpose

Mammalian expression of MERS coronavirus S protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

MERS-CoV_S (S MERS coronavirus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-sgTel

Plasmid

#122659

Purpose

Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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PE-Cas9

Plasmid

#174851

Purpose

Express PE-Cas9 in mammalian cells

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

PE-Cas9

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pKAd5f11pES-PmeI

Plasmid

#122698

Purpose

To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Type

Empty backbone

Use

Adenoviral

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP670-2-T2A-HO1

Plasmid

#197250

Purpose

Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP670-2-T2A-HO1

Expression

Worm

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pKAd5f11pABR-EPG

Plasmid

#122699

Purpose

It can be used to construct a recombinant virus carrying other transgene or an adenovector with modified fiber.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Insert

GFP

Use

Adenoviral

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.6

Plasmid

#197199

Purpose

Expresses the protein of BDFP1.6 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.6

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_CHHF_GPC

Plasmid

#207260

Purpose

Mammalian expression of Chapare hemorrhagic fever virus GPC protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZIBG2

Plasmid

#169458

Purpose

cell surface localized zinc sensor with ~ 300 nM Kd

Depositor

Huiwang Ai

Article

Chen et al Anal Chem. 2019 Oct 1;91(19):12212-12219

Insert

ZIBG2

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-AsCas12f-WT

Plasmid

#204635

Purpose

Expression of wild-type AsCas12f in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_SLEV_PrME

Plasmid

#207309

Purpose

Mammalian expression of St. Louis encephalitis virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

SLEV_PrME (POLY St. Louis encephalitis virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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