We narrowed to 17,207 results for: puro

1. pLentiCRISPR-v1-sgSHMT2

   Plasmid

   #217435

   Purpose

   Lentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

   Insert

   sgRNA targeting SHMT2 (SHMT2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMRX-IP-hWIPI3-3×FLAG

   Plasmid

   #215511

   Purpose

   Expresses FLAG tagged human WIPI3.

   Depositor

   Noboru Mizushima

   Article

   Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

   Insert

   WD-repeat protein interacting with phosphoinositides 3 (WDR45B Human)
   

   Use

   Retroviral

   Tags

   3×FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMRX-IPU-Venus-GS27

   Plasmid

   #228138

   Purpose

   Expresses Venus-GS27 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   GS27 (GOSR2 Human)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-IPU-Venus-Rab11

   Plasmid

   #228140

   Purpose

   Expresses Venus-Rab11 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   Rab11 (RAB11A Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIX401 APE1 WT

   Plasmid

   #227190

   Purpose

   Inducible lentiviral expression of WT APE1

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLIX401 APE1 SO

   Plasmid

   #227192

   Purpose

   Inducible lentiviral expression of SO (Slow Off) APE1

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   D283A D308A

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57-CMV-MAAP-SL01V6

   Plasmid

   #218249

   Purpose

   1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein Variant SL01V6 driven by CMV promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57-CMV-MAAP-SL01V9

   Plasmid

   #218250

   Purpose

   1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein Variant SL01V9 driven by CMV promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW57-CMV-MAAP-SL08V9

   Plasmid

   #218251

   Purpose

   1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRE3G-NS3/4A S139A

    Plasmid

    #186885

    Purpose

    Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    HCV NS3/4A protease with S139A mutation
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    S139A

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRE3G-WHG cGAS-HA

    Plasmid

    #186867

    Purpose

    Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    White-handed gibbon cGAS with C-terminal HA tag
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCW57-CMV-MAAP-SL01

    Plasmid

    #218248

    Purpose

    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein Variant SL01 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRXIP-GFP-PKD C1ab

    Plasmid

    #221755

    Purpose

    Stable expression of GFP-PKD C1ab in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    Prkd1(138-343) (Prkd1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 138-343

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-cT3G-cERP2-PRDM1

    Plasmid

    #222924

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    PRDM1 (PRDM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LentiCRISPRv2 EGFP sgRNA4

    Plasmid

    #164934

    Purpose

    Expresses EGFP sgRNA4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPRv2 NT sgRNA5

    Plasmid

    #164930

    Purpose

    Expresses Non-targeting sgRNA5 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA5 (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-cT3G-cERP2-ZGLP1

    Plasmid

    #222926

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZGLP1 (ZGLP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LT3GEPIR-WT1shRNA

    Plasmid

    #220560

    Purpose

    To inducibly knockdown WT1 or EWSR1::WT1 expression

    Depositor

    Sean Lee

    Article

    Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

    Insert

    WT1 shRNA (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G (TetOP) promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRX-IP-hWIPI2B-3×FLAG

    Plasmid

    #215510

    Purpose

    Expresses FLAG tagged human WIPI2B.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCALPS-Flag-Miwi-EGFP

    Plasmid

    #212575

    Purpose

    Lentiviral expression of Flag-tagged mouse Miwi in mammalian cells

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    Miwi (Piwil1 Mouse)
    

    Use

    Lentiviral

    Tags

    5x Flag and SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p667-UBC-MAFG-V5-miniTurbo

    Plasmid

    #207818

    Purpose

    C-terminal tagged MAFG for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    MAFG-V5-miniTurbo
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEF1a-mXRCC1pd-eGFP

    Plasmid

    #206035

    Purpose

    Mammalian expression of PAR-deficient mXRCC1pd coupled to eGFP under the control of a EF1a promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    mXRCC1pd
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S105A, S186A, K188A, S195A, S221A, S222A,S238A, S…

    Promoter

    Ef1a

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p663-UBC-miniTurbo-V5-MAFG

    Plasmid

    #207817

    Purpose

    N-terminal tagged MAFG for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-MAFG
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p663-UBC-miniTurbo-V5-ZNF746

    Plasmid

    #207819

    Purpose

    N-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-ZNF746 (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCW57.1-ZNF746-C571A

    Plasmid

    #207826

    Purpose

    Dox-inducible expression of ZNF746-C571A

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-C571A (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    C571A

    Promoter

    TRE

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p667-UBC-ZNF746-V5-miniTurbo

    Plasmid

    #207820

    Purpose

    C-terminal tagged ZNF746 for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746-V5-miniTurbo (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    V5-miniTurbo

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLVX-CALPAIN-7-mCherry, F98D

    Plasmid

    #180653

    Purpose

    Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CALPAIN-7
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GCACCCAUACCUUUACAUU

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LENTICRISPR-SNRPA_sgRNA1

    Plasmid

    #201622

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    SNRPA (SNRPA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LENTICRISPR-SNRPA_sgRNA2

    Plasmid

    #201623

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    SNRPA (SNRPA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX317-VRK2

    Plasmid

    #199641

    Purpose

    Expresses VRK2

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK2 (VRK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLX317-VRK2-K168E

    Plasmid

    #199642

    Purpose

    Expresses kinase-inactive VRK2 (K168E)

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK2 (VRK2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K168E

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc

    Plasmid

    #200127

    Purpose

    Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] P2A firefly luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc

    Plasmid

    #200128

    Purpose

    Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA(SA)] P2A firefly luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

    Plasmid

    #200126

    Purpose

    Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pXPR_003 sgGPAA1 guide 1

    Plasmid

    #193608

    Purpose

    GPAA1 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgGPAA1 guide 1 (GPAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pXPR_003 sgGPAA1 guide 2

    Plasmid

    #193609

    Purpose

    GPAA1 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgGPAA1 guide 2 (GPAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pXPR_003 sgAP2M1 guide 1

    Plasmid

    #193600

    Purpose

    AP2M1 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgAP2M1 guide 1 (AP2M1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pXPR_003 sgAP2M1 guide 2

    Plasmid

    #193601

    Purpose

    AP2M1 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgAP2M1 guide 2 (AP2M1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pXPR_003 sgFADD guide 1

    Plasmid

    #193589

    Purpose

    FADD knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgFADD guide 1 (FADD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pXPR_003 sgFADD guide 2

    Plasmid

    #193590

    Purpose

    FADD knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgFADD guide 2 (FADD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge