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mARG1 cassette 2

Plasmid

#134344

Purpose

Mammalian acoustic reporter gene 1 cassette 2 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAT28-proD/SaEGFP

Plasmid

#120321

Purpose

Bacterial eGFP labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

eGFP with Staphylococcus aureus codon optimization

Expression

Bacterial

Promoter

proD

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pNR220

Plasmid

#79541

Purpose

Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

TP901
BxbI
A118

Tags

His tag and LAA degradation tag

Expression

Bacterial

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pXT128_TwinStrep-SUMO-ShCas12k

Plasmid

#135524

Purpose

Protein expression construct for ShCast12k

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShCas12k

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

Plasmid

#162233

Purpose

Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody lag16lag2 and IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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p8.9 NdeltaSB

Plasmid

#132929

Purpose

Minimal HIV-1 packaging plasmid for gag and pol expression

Depositor

Jeremy Luban

Article

Berthoux et al J Virol. 2004 Nov;78(21):11739-50. doi:

Insert

gag and pol

Use

Lentiviral

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJLG039

Plasmid

#192982

Purpose

BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

Depositor

Clay Fuqua

Article

Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

Insert

T7 polymerase

Expression

Bacterial

Promoter

lac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rap2b

Plasmid

#118321

Purpose

Expression of human Rap2b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap2b (RAP2B Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJOE6227.1

Plasmid

#135078

Purpose

An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

up- and downstream regions of upp (upp Pseudomonas putida)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRS712

Plasmid

#213041

Purpose

CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

Depositor

Rebecca Shapiro

Article

Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP3665

Plasmid

#222351

Purpose

Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-CFP

Plasmid

#90103

Purpose

CFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

CFP

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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mARG1 cassette 3

Plasmid

#134345

Purpose

Mammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.

Depositor

Mikhail Shapiro

Article

Farhadi et al Science. 2019 Sep 27;365(6460):1469-1475

Insert

mARG 1 cassete 3

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKM492-Myc-His

Plasmid

#109283

Purpose

ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Myc-His

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHC79

Plasmid

#167551

Purpose

Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Type

Empty backbone

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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SeAvs3

Plasmid

#188791

Purpose

Avs3 from Salmonella enterica NCTC13175

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs3

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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p8266 LentiCRISPR v2 hygro sgNT-1

Plasmid

#193977

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub

Plasmid

#232577

Purpose

Express Ubiquitin tagged with an Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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C1-CFP-Lyn11

Plasmid

#155234

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lyn11 membrane targeted CFP. Membrane control

Tags

CFP

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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