We narrowed to 28,440 results for: REP

1. PB-TET-mSox2

   Plasmid

   #20908

   Purpose

   piggyBac vector with tet inducible mSox2

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMU-4

   Plasmid

   #172353

   Purpose

   CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

   Depositor

   Eriko Takano

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   D4-TheoR-cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   D4

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-SRT-Puro

   Plasmid

   #154884

   Purpose

   piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

   Depositor

   Joseph Dougherty, Rob Mitra

   Article

   Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

   Insert

   piggyBac SRT
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS mouse c-Myc

   Plasmid

   #14972

   Depositor

   Joan Massague

   Article

   Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

   Insert

   c-Myc (Myc Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Nucleotides 2578-3294

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pKB01-PssbB-mKate2

   Plasmid

   #69105

   Purpose

   Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mKate2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-tetO-Klf4

   Plasmid

   #19764

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. Luc-SIRT1 mutant 3'UTR

   Plasmid

   #20380

   Depositor

   Charles Lowenstein

   Article

   Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

   Insert

   SIRT1 (SIRT1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

   Promoter

   Available since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET a sfGCaMP6s

   Plasmid

   #100022

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfGCaMP6s
   

   Use

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNHEJ-RPG

    Plasmid

    #85931

    Purpose

    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

    Depositor

    Zhiying Zhang

    Article

    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

    Insert

    pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
    

    Use

    Dual-reporter surrogate

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid

    #102895

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV Gab1(PRD)-mCherry-HOTag3

    Plasmid

    #190026

    Purpose

    Transiently expresses CluMPS for reporting on EML4-ALK oncogene clusters in mammalian cells.

    Depositor

    Lukasz Bugaj

    Article

    Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

    Insert

    Gab1(PRD) CluMPS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSSA-RPG

    Plasmid

    #85932

    Purpose

    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

    Depositor

    Zhiying Zhang

    Article

    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

    Insert

    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    

    Use

    Dual-reporter surrogate

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Promoter

    Pol II readthrough transcription after integration

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS416-Aga1p-FAPB2.3.6L1TAG

    Plasmid

    #158148

    Purpose

    Yeast display reporter for non-display yeast strains (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first position of th…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Oct4-Cr1

    Plasmid

    #66989

    Purpose

    Expresses gRNA that targets stop codon for OCT4

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    gRNA for Crispr targeting the OCT4 stop codon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pSSA-PMG (pXK-1649)

    Plasmid

    #208857

    Purpose

    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. sg2.0 (PP7) Locus#2

    Plasmid

    #101155

    Purpose

    gRNA with two PCP binding sites

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    Locus #2(tandem repeat)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ca-LiPPI-CLAPon1.0 (APEX)

    Plasmid

    #213408

    Purpose

    Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

    Depositor

    Wenjing Wang

    Article

    Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

    Insert

    MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pPB-CAG-Flag3-pG-mNanog-pgk-hph

    Plasmid

    #74911

    Purpose

    Mammalian expression of Flag tagged mNanog

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. Luc-Sox2-3'UTR

    Plasmid

    #63895

    Purpose

    Luciferase reporter of mouse wildtype Sox2 3'UTR

    Depositor

    Lin He

    Article

    Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

    Insert

    SRY (sex determining region Y)-box 2 (Sox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pEF-XMAS-2xStop

    Plasmid

    #164412

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRS415-Cas9-VPR

    Plasmid

    #163971

    Purpose

    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Insert

    Cas9-VPR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. KA1396_pGL4-UBCmin-luc2/Klf4-E'

    Plasmid

    #124187

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Ef1a-QCP-3xBFP

    Plasmid

    #158206

    Purpose

    Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    QCP under the Elfa promoter and three BFP tags
    

    Use

    Synthetic Biology

    Tags

    3xBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Elfa

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPA-RL-FL

    Plasmid

    #78863

    Purpose

    DULIP Tandem Construct used for data normalization

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    Firefly
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pSK260

    Plasmid

    #129252

    Purpose

    dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35 NM yTRAP sensor reporting on mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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