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We narrowed to 14,439 results for: eng

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Showing: 5761 - 5780 of 14439 results

  4. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid
    #176262
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176262-plasmid-map-sequence-id-346962

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  9. pEF1a-T2-CeNLuc

    Plasmid
    #135948
    Purpose
    Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.
    Depositor
    Antonio Amelio
    Publication
    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CeNLuc LumiFluor
    Tags
    T2 mitochondrial localization sequence
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    135948-plasmid-map-sequence-id-271491

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  10. pCEP4-Q769gp160

    Plasmid
    #100928
    Purpose
    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (Q769.d22) Env
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100928-plasmid-map-sequence-id-190002

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  11. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid
    #236007
    Purpose
    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835Y substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236007-plasmid-map-sequence-id-469607

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  12. pStreptagIIx2 Alfatag 3C InsR EABR

    Plasmid
    #234996
    Purpose
    For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Publication
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    InsR-EABR (INSR Human)
    Tags
    2xStreptag-II, Alfatag, HRV 3C site
    Expression
    Mammalian
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234996-plasmid-map-sequence-id-468745

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  15. FRB-GFP-Gab2(Y604F/Y634F)

    Plasmid
    #188659
    Purpose
    FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe
    Depositor
    Andrei Karginov
    Publication
    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GRB associated binding protein 2 (Gab2 Mouse)
    Tags
    FRB and GFP
    Expression
    Mammalian
    Mutation
    Y604F and Y634F
    Promoter
    CMV
    Available since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    188659-plasmid-map-sequence-id-369578

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  17. pMP926

    Plasmid
    #135001
    Purpose
    m6A Reader-Writer. Propagates Gm6ATC along GATC arrays as well as across cell generations. pUBC-DpnI(aa146-254)-3xFLAG-Dam(R95A) pGK-HygroR
    Depositor
    Ahmad Khalil
    Publication
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pUBC-DpnI(aa146-254)-3XFLAG-Dam(R95A) pGK-HygroR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG, Dam(R95A), and DpnI(aa146-254)
    Expression
    Mammalian
    Mutation
    DpnI domain truncated to aa146-254. Dam protein m…
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    135001-plasmid-map-sequence-id-267929

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  20. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Publication
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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Showing: 5761 - 5780 of 14439 results