We narrowed to 23,593 results for: promoter

1. pX459-sgRNA1-CTCF-prom

   Plasmid

   #195103

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against CTCF promoter
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIK208

   Plasmid

   #65630

   Purpose

   sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP in pDBEcR-pIND(SP1)-DD/Bla

   Plasmid

   #201559

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Promoter

   EF1a and pIND(SP1)

   Available Since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP in pDBEcR-pIND(SP1)-W/Bla

   Plasmid

   #201558

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc and FLAG-EGFP

   Expression

   Mammalian

   Promoter

   EF1a and pIND(SP1)

   Available Since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

   Plasmid

   #110830

   Purpose

   Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   AZGP1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   T2A and dTomato

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1 PIK3R1-Ni

   Plasmid

   #17744

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

   Insert

   PIK3R1-Ni (PIK3R1 Human)
   

   Expression

   Insect

   Mutation

   Partial cDNA

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA

   Plasmid

   #178100

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-26 (GB1001)

   Plasmid

   #68208

   Purpose

   Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtU6-26 promoter
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PliaI

   Plasmid

   #55177

   Purpose

   Bacitracin-inducible promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   liaI promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   liaI

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330 RBP1 gRNA

    Plasmid

    #165593

    Purpose

    Insertion of RBP1 degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    RBP1 (Polr2a Mouse)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

    Plasmid

    #124546

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
    

    Expression

    Mammalian

    Mutation

    WT, WT

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-Cdh1

    Plasmid

    #28127

    Depositor

    Pumin Zhang

    Article

    Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

    Insert

    fzr1 (FZR1 Human)
    

    Use

    Mouse Targeting and Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pVRc22_4450

    Plasmid

    #49741

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS22_4450
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. PliaG

    Plasmid

    #55174

    Purpose

    Constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    LiaG promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-rap1-K9R

    Plasmid

    #84527

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    none

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

16. REEP5-2Strep

    Plasmid

    #210350

    Purpose

    Expresses REEP5 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    REEP5 (REEP5 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-β-arrestin2 RRK/Q

    Plasmid

    #38261

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Arg233, Arg237, and Lys251 converted to Glutamines

    Promoter

    CMV

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pLV-rtTA-zeo

    Plasmid

    #183233

    Purpose

    Lentiviral vector expressing the rtTa gene

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    rtTA-T2A-Zeo
    

    Use

    Lentiviral

    Promoter

    UBC

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

    Plasmid

    #166413

    Purpose

    express hPOT1 ΔOB in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    Protection Of Telomeres 1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    ΔOB deleted 125 a.a

    Promoter

    ef1-a

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiCRISPRv2puro-Tbxa2r

    Plasmid

    #170304

    Purpose

    A knock-out vector for the mouse Tbxa2r

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A gRNA targeting the mouse Tbxa2r gene.
    

    Use

    CRISPR and Lentiviral

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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