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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,572 results for: GEM

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Showing: 1121 - 1160 of 2572 results

  4. mCit-4aa-SNAP25B

    Plasmid
    #53235
    Purpose
    Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53235-plasmid-map-sequence-id-421058

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  6. mCer-4aa-VAMP2

    Plasmid
    #53233
    Purpose
    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. VAMP2-12aa-mCit

    Plasmid
    #53237
    Purpose
    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    12aa-linker and mCitrine
    Expression
    Mammalian
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid
    #52495
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Science. 2014 Mar 27.
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Mutation
    E90R, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52495-plasmid-map-sequence-id-81430

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  33. mCer-Vamp2(qv/fw)

    Plasmid
    #53238
    Purpose
    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Mutated Q76 to V; F77 to W.
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1121 - 1160 of 2572 results