We narrowed to 1,178 results for: POLI

1. DCT.VV.Orf164

   Plasmid

   #12852

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf164
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-mSpata33 (I81A)-PA

   Plasmid

   #179131

   Purpose

   Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   spermatogenesis associated 33 (Spata33 Mouse)
   

   Use

   Tags

   PA

   Expression

   Mammalian

   Mutation

   Changed Isoleucine 81 to Alanine

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mSpata33 (P79A)-PA

   Plasmid

   #179130

   Purpose

   Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   spermatogenesis associated 33 (Spata33 Mouse)
   

   Use

   Tags

   PA

   Expression

   Mammalian

   Mutation

   Changed Proline 79 to Alanine

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mSpata33 (I83A)-PA

   Plasmid

   #179132

   Purpose

   Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   spermatogenesis associated 33 (Spata33 Mouse)
   

   Use

   Tags

   PA

   Expression

   Mammalian

   Mutation

   Changed Isoleucine 83 to Alanine

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_6xHis-PARP1-G871L

   Plasmid

   #174798

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G871L (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G871L, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1-A870L

   Plasmid

   #174797

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A870L (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A870L, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28_6xHis-PARP1-K893I

   Plasmid

   #174799

   Purpose

   Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-K893I (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K893I, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFAB1907

   Plasmid

   #47857

   Purpose

   BIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   BBa_B1006 U10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added two extra U at the end of Bba_1006 poly-U t…

   Promoter

   pLTetO12

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ10704

   Plasmid

   #183957

   Purpose

   U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   SKSKSO array
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ10871

    Plasmid

    #183962

    Purpose

    U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    dCas12a(wildtype)
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG and U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR221-BNT162b2ΔUTR

    Plasmid

    #171214

    Purpose

    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

    Depositor

    Liang Wei Wang

    Article

    Low et al medRxiv 2021.04.27.21256151

    Insert

    BNT162b2ΔUTR
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Kozak sequence changed from CCCGCCACC to GGCTGCAC…

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTEF:ATP

    Plasmid

    #92179

    Purpose

    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    

    Use

    Integration and expression into yeast.

    Tags

    Yeast codon optimized mseCFP (donor) and cp173-mV…

    Expression

    Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-E988K

    Plasmid

    #173945

    Purpose

    Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-E988K (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A, E988K

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-hPPP3CA-FLAG

    Plasmid

    #179134

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Use

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAaePUb::Cas9-2A-Neo

    Plasmid

    #176680

    Purpose

    Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDUET-1-alpha-synuclein-mCerulean3-His6

    Plasmid

    #110061

    Purpose

    For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    6 x His and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2R3a-nosT

    Plasmid

    #71272

    Purpose

    Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    nopaline synthase terminator
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_6xHis-PARP1-CAT-L713F

    Plasmid

    #173944

    Purpose

    Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CAT-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge