We narrowed to 6,273 results for: nls

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mCherry-NLS-mCherry

Plasmid

#231890

Purpose

mCherry-tagged nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

mCherry-SV40 NLS-mCherry

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ GCamp6-3NLS

Plasmid

#165401

Purpose

Expresses a nuclear localized genetically encoded calcium reporter.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

GCamp6

Expression

Mammalian

Promoter

SP6

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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GANLS-DEVD-BNES

Plasmid

#50835

Purpose

Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A and ddRFP B

Tags

NES sequence LALKLAGLDIGS and triplicated NLS seq…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-PCNA-19-SV40NLS-4

Plasmid

#55320

Purpose

Localization: Nucleus, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

3xSV40 NLS and mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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PS Intein NLS-NES

Plasmid

#242021

Purpose

Blue-light-induced reconstitution of mCherry and its relocalization to the nucleus.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

PS Intein NLS-NES

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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epB_UbC_CymRV5-nls-GFP_DEx2

Plasmid

#119906

Purpose

ePiggyBac mammalian expression plasmid for CymR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-SB-NLS

Plasmid

#228870

Purpose

PE7 connected to MLH1-SB-NLS via 2A sequence

Depositor

Sangsu Bae

Article

Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

Insert

PE7-2A-MLH1-SB-NLS

Use

CRISPR

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-parallel1-NLSH2BCas9

Plasmid

#112065

Purpose

Expression and purification of NLSH2BCas9 for fungal gene editing

Depositor

Jeff Coleman

Article

Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

Insert

NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger

Use

CRISPR

Tags

His tag

Expression

Bacterial

Mutation

Cas9 was codon optimized for Aspergillus niger

Promoter

promoter in vector

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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KillerRed-LacI-NLS

Plasmid

#215102

Purpose

Expresses KillerRed-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

KillerRed-LacI-NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PKI nls

Plasmid

#118301

Purpose

Expression of PKIa

Depositor

Philip Stork

Article

Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

Insert

PKI nls (aa 2-36)

Tags

Flag and GFP

Expression

Mammalian

Mutation

inserting 2xnls

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PV-NLS-GFP

Plasmid

#17300

Depositor

Anton Bennett

Article

Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

Insert

PV-GFP-NLS (Pvalb Rat)

Tags

Myc and NLS

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

Plasmid

#52421

Purpose

Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

Depositor

Bertrand Collet

Insert

Fusion NLS-EGFP-P2A-Cherry-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Expression

Worm

Promoter

rab-3

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-polyQ23-NLS

Plasmid

#231891

Purpose

Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q23-NLS (HTT Human)

Tags

EGFP and SV40 NLS

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-opCas12a-6xNLS

Plasmid

#209022

Purpose

Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

opCas12a-6xNLS-Flag-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

FLAG and MYC

Expression

Mammalian

Mutation

E174R/S542R

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Article

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Tags

RFP

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-TORCAR-NLS

Plasmid

#64931

Purpose

Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR-NLS (EIF4EBP1 Synthetic, Human)

Tags

Cerulean, Nuclear localization signal (NLS), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+ EGFP-3NLS

Plasmid

#165400

Purpose

Expresses nuclear localized EGFP

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

EGFP

Expression

Mammalian

Promoter

SP6

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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