We narrowed to 6,177 results for: nls

1. CMV-NLS-AcrIIA4

   Plasmid

   #113037

   Purpose

   Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-NLS-actin

   Plasmid

   #118380

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   NLS

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PV-NLS-GFP

   Plasmid

   #17300

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-GFP-NLS (Pvalb Rat)
   

   Tags

   Myc and NLS

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. epB_CAG_TetRFlag-nls-GFPx2_DEx2

   Plasmid

   #119910

   Purpose

   ePiggyBac mammalian expression plasmid for TetR-GFP fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   TetRFlag-nls-GFPx2
   

   Expression

   Mammalian

   Available Since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-2NLS-Cas9

   Plasmid

   #229773

   Purpose

   Expresses Cas9 driven by a CMV promoter

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

   Insert

   SpCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP

   Plasmid

   #32628

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Sce
   

   Use

   Lentiviral

   Available Since

   Dec. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-NLS-TurboID

   Plasmid

   #163849

   Purpose

   In vitro transcription of nls-TurboID. Parton lab clone JST

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID
   

   Use

   In vitro transcription of rna

   Tags

   nls

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB_SBE4:mScarlet-I-NLS

   Plasmid

   #154961

   Purpose

   encodes transcriptional reporter of SMAD2/3

   Depositor

   Joshua Knowles, Ewa Bielczyk-Maczynska

   Article

   Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

   Insert

   mScarlet
   

   Use

   Piggybac

   Tags

   NLS-PEST

   Expression

   Mammalian

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-NLS-R-GECO

   Plasmid

   #32462

   Purpose

   Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   R-GECO1.0
   

   Tags

   NLS sequence DPKKKRKV

   Expression

   Mammalian

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-CMV-nlsCRE

    Plasmid

    #12265

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frb-NLS-mCherry

    Plasmid

    #217613

    Purpose

    part B of SPARK-OFF

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    Frb-NLS-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+ GCamp6-3NLS

    Plasmid

    #165401

    Purpose

    Expresses a nuclear localized genetically encoded calcium reporter.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    Nuclear Localization Signal
    

    Use

    Avian, xenopus, zebrafish

    Tags

    GCamp6

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWT35S-mCherry-NLS

    Plasmid

    #194049

    Purpose

    Transient expression of mCherry-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    mCherry
    

    Tags

    SV40 (Nuclear localization signal)

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR_CMV_MeltNLS/NES-40-mCherry

    Plasmid

    #234066

    Purpose

    Encodes a protein that localizes to nucleus under the control of a Melt variant with a switching temperature of 40°C

    Depositor

    Lukasz Bugaj

    Article

    Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

    Insert

    MeltNLS/NES-40-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. V5-BS2-NLS (Nucleoplasmin)

    Plasmid

    #220342

    Purpose

    Mammalian expression vector BS2 in nucleus

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Tags

    NLS (Nucleus localization sequence) and V5 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid

    #188523

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    3XNLS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-AKAR4-NLS

    Plasmid

    #138217

    Purpose

    Nuclear-targeted FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    AKAR4-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. NLS-SpCas9mSA-His

    Plasmid

    #182951

    Purpose

    Express SpCas9mSA in bacteria

    Depositor

    Wen Xue

    Article

    Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

    Insert

    NLS-SpCas9mSA-His
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBCCP-GFP-NLS

    Plasmid

    #173923

    Purpose

    Fluorescent labeling of the nuclear envelope

    Depositor

    Shinji Sueda

    Article

    Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

    Insert

    Biotin carboxyl carrier protein, AcGFP1
    

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-EF1aL-nlsGFP-W

    Plasmid

    #126688

    Purpose

    Constitutive, ubiquitous, nuclear-localized GFP expression.

    Depositor

    Darrell Kotton

    Article

    Tang et al Nat. Phys. 18, 1371–1378 (2022).

    Insert

    nlsGFP
    

    Use

    Lentiviral

    Tags

    6xHIS and NLS

    Promoter

    EF1aL

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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