We narrowed to 26,944 results for: sta

1. qTAG-C-Solo-mStayGold-CANX

   Plasmid

   #227280

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold Tag (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mStayGold-EFS-Zeo

   Plasmid

   #227258

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Solo-mStayGold-TUBA1B

   Plasmid

   #227327

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2Apuro-Stargazin-mEGFP-iLID

   Plasmid

   #178529

   Purpose

   Expresses Stargazin-mEGFP-iLID in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   Stargazin-mEGFP-iLID
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-Solo-mStayGold-COX8A

   Plasmid

   #227309

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-Stargazin-mEGFP-iLID

   Plasmid

   #178523

   Purpose

   Expresses Stargazin-mEGFP-iLID in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   Stargazin-mEGFP-iLID
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-Solo-mStayGold-LAMP1

   Plasmid

   #227322

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-VIM

   Plasmid

   #227302

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   VIM Homology Arms flanking a mStayGold Tag (VIM Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Venus-StagRFP-AktAR

   Plasmid

   #173020

   Purpose

   Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Venus-StagRFP-AktAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-mStayGold-Puro-CLTC

    Plasmid

    #227314

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-Solo-mStayGold-PEX3

    Plasmid

    #227304

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the PEX3 locus. For peroxisome visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PEX3 (Addgene #227303)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PEX3 Homology Arms flanking a mStayGold Tag (PEX3 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. qTAG-N-Solo-mStayGold-RAB7A

    Plasmid

    #227298

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the RAB7A locus. For endosome visualization. To be co-transfected with sgRNA plasmid px330-RAB7A (Addgene #227297)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    RAB7A Homology Arms flanking a mStayGold Tag (RAB7A Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Stat3 S727A pRc/CMV

    Plasmid

    #8708

    Depositor

    Jim Darnell

    Article

    Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

    Insert

    Stat3 S727A (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S727A (exhibits o serine phosphorylation either c…

    Promoter

    Available since

    Aug. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. Stat1 alpha pRC/CMV

    Plasmid

    #8690

    Depositor

    Jim Darnell

    Article

    Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

15. His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)

    Plasmid

    #222991

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUASTattB-dALFANb(3mut)-mGreenLantern

    Plasmid

    #219662

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3puro-mGFP-FXR1a-sh5Resistant

    Plasmid

    #225448

    Purpose

    Express mEGFP-fusion protein of FXR1 isoform a with shRNA5-resistant silent mutation

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FXR1 isoform a (FXR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST39-Hrs-STAM1 core

    Plasmid

    #21497

    Depositor

    James Hurley

    Article

    Ren et al Structure. 2009 Mar 11. 17(3):406-16.

    Insert

    pST39- Hrs(398-514)-His/STAM1(301-387) (STAM, HGS Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

19. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid

    #227307

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BAP1-C91A (siRNA resistant)

    Plasmid

    #108438

    Purpose

    Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C91A: c.271T>G, c.272G>C; others: c.1641C&g…

    Promoter

    CMV

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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