We narrowed to 18,395 results for: Tec

1. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EYFP-Clathrin-19

   Plasmid

   #56584

   Purpose

   Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pCellFree_G03 ISG15

   Plasmid

   #67054

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pJL1-EPA-DNNNS-DQNRT

   Plasmid

   #128397

   Purpose

   In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Exotoxin A
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   242DNNNS246 and 384DQNRT388 engineered glycosylat…

   Promoter

   T7

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGWB527

   Plasmid

   #74869

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDUAL_2_T5

   Plasmid

   #155175

   Purpose

   see pDUAL_T5: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNAS1b split mCherry 14-3-3β

   Plasmid

   #111880

   Purpose

   Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)

   Depositor

   Jesse Rinehart

   Article

   Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

   Type

   Empty backbone

   Use

   Tags

   Split mCherry system: 14-3-3β fused to C-terminal…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV5-Flag-RapR-p38

   Plasmid

   #25935

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

   Insert

   RapR-p38 (Mapk14 Human, Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. dTomato_LD 0_CS G_tTA-BFP

   Plasmid

   #58871

   Purpose

   MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB525

    Plasmid

    #74867

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TAL2344

    Plasmid

    #36764

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    PTEN-TALEN_Left (Pten Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TAL2345

    Plasmid

    #36765

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    PTEN-TALEN_Right (Pten Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Use

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs-ms-Sox1

    Plasmid

    #50778

    Purpose

    retroviral expression of mouse Sox1

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

    Insert

    Sox1 (Sox1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03 GATA1

    Plasmid

    #67068

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pUC57-GSX2-tTA-BleoR

    Plasmid

    #161748

    Purpose

    Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    Advanced tTA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    endogenous GSX2 promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. mVenus-CENPB-N-22

    Plasmid

    #56325

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    CENPB (CENPB Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGWB424

    Plasmid

    #74818

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TAL2362

    Plasmid

    #36775

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    EZH2-TALEN_Left (Ezh2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only