We narrowed to 30,619 results for: ica

1. CRISPR_TRAC

   Plasmid

   #164993

   Purpose

   Expression of gRNA targeting TCRalpha constant locus

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against TRAC
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_mcherry

   Plasmid

   #167862

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. miR-vector-RFP pCDNA

   Plasmid

   #97143

   Purpose

   for expression of miRNA and TagRFP

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Type

   Empty backbone

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pICH47732:PtFCP:ShBle

   Plasmid

   #104893

   Purpose

   Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

   Depositor

   Thomas Mock

   Article

   Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

   Insert

   ShBle
   

   Use

   Phaeodactylum; golden gate assembly

   Tags

   PtFCP promoter and terminator

   Expression

   Mutation

   Promoter

   Available since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Nano-lantern(cAMP-0.4)/pcDNA3

   Plasmid

   #53593

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. human p53-(310-360)

   Plasmid

   #24872

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains only the dimeric oligomerization domain …

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

7. GFP-NPM NLS1/2D

   Plasmid

   #13287

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   NPM (NPM1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of aa134-aa142, aa150-aa155

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pRc/CMV HA TrkB-T1

   Plasmid

   #39980

   Depositor

   Yves-Alain Barde

   Article

   Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

   Insert

   TrkB.T1 (Ntrk2 Rat)
   

   Use

   Tags

   BM40 signal sequence and HA

   Expression

   Mammalian

   Mutation

   TrkB.T1 variant; endogenous signal sequence was r…

   Promoter

   CMV

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 FLIPE-100u

   Plasmid

   #13543

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

    Plasmid

    #199347

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-3C

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. H2A-mTurquoise-RHOG-QL-deltaCAAX

    Plasmid

    #176096

    Purpose

    Constitutively active, nuclear localized RhoG

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOG (RHOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    QL

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFLAG-ING2

    Plasmid

    #13295

    Depositor

    Curtis Harris

    Article

    Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

    Insert

    ING2 (INGX Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pKF257

    Plasmid

    #52533

    Purpose

    expresses I-SceI nuclease in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Tags

    HA and NLS

    Expression

    Insect

    Mutation

    Promoter

    tubulin

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

    Plasmid

    #104116

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    QuasAr3-Citrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SHC003BSD

    Plasmid

    #133300

    Purpose

    The puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET15b/BOA

    Plasmid

    #82041

    Purpose

    For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

    Depositor

    Angela Gronenborn

    Article

    Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

    Insert

    Burkholderia oklahomensis agglutinin
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSB700 lenti gRNA EBFP2

    Plasmid

    #167905

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046871

    Plasmid

    #145724

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3 SWS cone opsin

    Plasmid

    #109363

    Purpose

    Human SWS cone opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    SWS cone opsin (OPN1SW Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046731

    Plasmid

    #145633

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pBTK1003 (pSL2)

    Plasmid

    #190998

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CP25

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. optGluCl beta Y182F ECFP

    Plasmid

    #15105

    Depositor

    Henry Lester

    Article

    Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

    Insert

    GluCl beta (glc-2 Nematode)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA, Y182F

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-NPM NESM-NLS1D

    Plasmid

    #13288

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L100A, L102A, deletion of aa134-aa142

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pX330-sgRNA_Dgcr8_1

    Plasmid

    #73525

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

25. JB20

    Plasmid

    #128912

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    Rab11AQL-BirA*-HA-MAO (RAB11A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBWB424

    Plasmid

    #140638

    Purpose

    RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.hSynap.iGABASnFR.R205A

    Plasmid

    #112162

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.R205A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    R205A (no GABA binding)

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGBW-m4046833

    Plasmid

    #145649

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. NAX_CAGGS_CAS9_m4_Puro

    Plasmid

    #167871

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-N1-mt-ro2GFP

    Plasmid

    #82408

    Purpose

    Expression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cells

    Depositor

    S. James Remington

    Article

    Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

    Insert

    ro2GFP
    

    Use

    Tags

    leader sequence of the E1α subunit of pyruvate de…

    Expression

    Mammalian

    Mutation

    C48S/F64L/S147C/Q204C

    Promoter

    CMV

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GST-Cav FL (1-178)

    Plasmid

    #22445

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

    Insert

    Caveolin1(1-178)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pc-GAKIN-Ha

    Plasmid

    #188246

    Purpose

    Express GAKIN in mammalian cells with Ha tag

    Depositor

    Joel Pomerantz

    Article

    Lamason et al Mol Cell. 2010 Dec 10;40(5):798-809. doi

    Insert

    GAKIN (KIF13B Human)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pME18S-3HA-mULK1

    Plasmid

    #22896

    Depositor

    Masaaki Muramatsu

    Article

    Yan et al Biochem Biophys Res Commun. 1998 May 8.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

34. Bov.hsc70.RSET.FL.wt(NarI)

    Plasmid

    #12532

    Depositor

    David McKay

    Article

    Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

    Insert

    bovine 70 kDa heat shock cognate protein full length wild type
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMSCV-FKBP-empty

    Plasmid

    #221239

    Purpose

    Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    FLAG-4xFKBP scaffold
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pB80-2xFKBP-L-mCherry-CAAX

    Plasmid

    #193713

    Purpose

    Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    2xFKBP-L-mCherry-CAAX
    

    Use

    Tags

    2xFKBP-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-ING2

    Plasmid

    #13294

    Depositor

    Curtis Harris

    Article

    Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

    Insert

    ING2 (INGX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pET28a-cMSPdH45

    Plasmid

    #199767

    Purpose

    Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH45
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUAST cyto roGFP2-Orp1

    Plasmid

    #64996

    Purpose

    for expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDNA3-ySet2

    Plasmid

    #51698

    Purpose

    Expresses full length yeast Set 2 gene in a mamalian expression vector

    Depositor

    Tom Misteli

    Article

    Luco et al Science. 2010 Feb 19;327(5968):996-1000.

    Insert

    yeast Set2 (SET2 Budding Yeast)
    

    Use

    Tags

    C-terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only