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pYTRW11K_0S5

Plasmid

#177282

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with SmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PaadA-aadA

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW20K_0Ti1

Plasmid

#177289

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Ptet-tetA(C)

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -2

Plasmid

#173891

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-598)_quadruple_mutant

Plasmid

#135441

Purpose

Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

L535A, L539A, L546A, M549A

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-HDAC4*

Plasmid

#71402

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4* (HDAC4 Human)

Expression

Mammalian

Mutation

After aa126, insertion leads to reading frame shi…

Promoter

CAG

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
1GFP/RNase H1 D210N

Plasmid

#174448

Purpose

Express GFP-tagged RNase H1 D210N in E. coli.

Depositor

Karlene Cimprich

Article

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Tags

His-tag, GFP

Expression

Bacterial

Mutation

D210N

Promoter

T7 promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLP-PIGA (pLP140)

Plasmid

#168461

Purpose

Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

Depositor

Jef Boeke, Matthew Maurano

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Type

Empty backbone

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGolt-mCherry / pGolt3-mCherry

Plasmid

#73297

Purpose

Visualization of Dendritic Golgi Satellite

Depositor

Michael Kreutz

Article

Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1

Inserts

ER export signal of Scap
ER export signal of Scap
TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)

Tags

ER export and mCherry

Expression

Mammalian

Promoter

CMV immediate early promoter

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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NST1_pOpOn2.1

Plasmid

#228065

Purpose

Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

Depositor

Charles Anderson

Article

Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

Insert

NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)

Use

Dexamethasone inducible vector

Expression

Plant

Promoter

CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-GCaMP6F

Plasmid

#137122

Purpose

Intersectional viral expression of GCaMP6F in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV5 and AAV8

Insert

Con/Fon-GCaMP6F

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-PalmGRET

Plasmid

#158221

Purpose

BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

PalmGFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon DREADD Gi-mCherry

Plasmid

#177669

Purpose

Expresses DREADD hM4D (Gi) only in Flp expressing cells and not in Cre expressing cells

Depositor

Karl Deisseroth

Article

Knoedler et al Cell. 2022 Feb 17;185(4):654-671.e22. do

Insert

DREADD Gi-mCherry

Use

AAV, Cre/Lox, and Mouse Targeting ; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-FLPo-Myc

Plasmid

#124641

Purpose

AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

Depositor

David Dupret

Article

Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

Insert

FLPo

Use

AAV and Cre/Lox

Tags

myc tag

Expression

Mammalian

Promoter

EF1a

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-mCherry

Plasmid

#137134

Purpose

Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV-MRLC1-mRuby2-IRES-PuroR

Plasmid

#103031

Purpose

Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

MRLC1 (MYL9 Human)

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Fon-mCherry

Plasmid

#137132

Purpose

Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-mCherry

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-mitoGFP-DIO

Plasmid

#174112

Purpose

Cre-dependent expression of mitochondria-targeted Green Fluorescent Protein under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Has Service

AAV2

Insert

mitoGFP (COX8A Human)

Use

AAV

Tags

Cox8 targeting sequence and GFP

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

Plasmid

#55646

Purpose

Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-EYFP

Plasmid

#137162

Purpose

Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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