We narrowed to 146,921 results for: ins;

1. pBABE human IRS-1 CAAX

   Plasmid

   #11237

   Depositor

   Ronald Kahn

   Article

   Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Retroviral

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   C-terminal prenylation motif makes IRS-1 localize…

   Promoter

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pTox-EGFPsite1_NGA-PAM (BPK754)

   Plasmid

   #181747

   Purpose

   p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)
   

   Use

   Selection plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-agrBD-IV

   Plasmid

   #53440

   Purpose

   Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrBD locus (with D29Y mutation in agrD)
   

   Use

   Synthetic Biology

   Tags

   V5 epitope, 6x HIS (not expressed on agrB or agrD…

   Expression

   Bacterial

   Mutation

   D to Y mutation of residue 29 of the agrD gene (c…

   Promoter

   T7-lac

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBSSK p85 alpha (delta SH3, iSH2)

   Plasmid

   #13433

   Depositor

   Ronald Kahn

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

   Insert

   p85 alpha (delta SH3, iSH2) (PIK3R1 Human)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Flag tags replacing both the SH3 and iSH2 domains.

   Promoter

   Available Since

   Nov. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

5. HOXC4 (human) MSCV

   Plasmid

   #8546

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXC4 (HOXC4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Contains improved ribosomal binding site and NcoI…

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pFastBac1-OSF-TRIM5alpha(human)(R332P)

   Plasmid

   #79037

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human R332P) (TRIM5 Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Changed Arg 332 to Pro

   Promoter

   polyhedrin

   Available Since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX p85b(NC)-SH2

   Plasmid

   #46468

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   p85b(NC) (PIK3R2 Bovine)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 311-717

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Nck2-SH2

   Plasmid

   #46458

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   NM_001004720.2 (NCK2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 274-380

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. DYNLL1

   Plasmid

   #132530

   Purpose

   Human LC8-1, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLL1 (DYNLL1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. DYNC2LI1

    Plasmid

    #132527

    Purpose

    Human LIC3, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNC2LI1 (DYNC2LI1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. DYNLRB1

    Plasmid

    #132533

    Purpose

    Human Rbl-1, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLRB1 (DYNLRB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Tem6-SH2

    Plasmid

    #46523

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Tem6 (TNS3 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1171-1291

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

    Plasmid

    #174639

    Purpose

    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-GFP-ppKinVIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-GFP

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

    Plasmid

    #159658

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Vrap-SH2

    Plasmid

    #46531

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Vrap (SH2D2A Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 93-192

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXylan

    Plasmid

    #36301

    Depositor

    Jay Keasling

    Article

    Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

    Insert

    pXylan
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Two promoters from the E coli genome drive two di…

    Available Since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX BRDG1-SH2

    Plasmid

    #46410

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    BRDG1 (STAP1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 167-283

    Promoter

    Tac

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFlg113

    Plasmid

    #101020

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    cmv

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX Shb-SH2

    Plasmid

    #46485

    Depositor

    Bruce Mayer, Michael Welsh

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Shb (SHB Human)
    

    Use

    Tags

    GST, HA, and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 401-508

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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