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tetO-FUW-eGFP-RHOA-Q63L

Plasmid

#73081

Purpose

Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63L

Depositor

Andrew Putnam

Article

Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

Insert

RHOA-Q63L (RHOA Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-gRNA-EF1a-EGFP-CAAX

Plasmid

#167936

Purpose

gRNA entry vector

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

U6-gRNA backbone

Use

Lentiviral

Promoter

U6

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-EGFP-T2A-RPL22-1xFlag

Plasmid

#170323

Purpose

Expresses FLAG and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSFV_eGFP-P2A-luc2pre (3'UTR)_RLEloop

Plasmid

#240255

Purpose

Attenuated PROTEUS vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

EGFP-P2A-firefly luciferase

Expression

Mammalian

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFP

Plasmid

#244208

Purpose

Mammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-4xSID

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-EGFP-WPRE-pA

Plasmid

#37091

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

d1eGFP

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-EGFP-GPI-stop-L2

Plasmid

#186355

Purpose

Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)

Use

Expression of a fluorescent membrane marker

Tags

EGFP

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-U6/TO-shEsr1-CMV-TetR-eGFP

Plasmid

#233298

Purpose

DOX-inducible U6 driven shRNA against mouse Esr1

Depositor

Dayu Lin

Article

Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

Insert

Esr1 shRNA (Esr1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

Plasmid

#69100

Purpose

URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

Depositor

David Botstein

Inserts

yZ3EV cassette
Z3 promoter
yEGFP

Expression

Yeast

Available Since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHIV-ODD-EGFP-IRES-dTomato

Plasmid

#87218

Purpose

lentiviral expression of ODD-EGFP

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a ODD domain (HIF1A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

ODD domain

Promoter

EF1

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pL-U6-sgRNA-SFFV-Puro-P2A-EGFP

Plasmid

#175037

Purpose

Lentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Sp sgRNA scaffold

Use

Lentiviral

Promoter

U6

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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tetO-FUW-eGFP-RHOA-T19N

Plasmid

#73082

Purpose

Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-T19N

Depositor

Andrew Putnam

Article

Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

Insert

RHOA-T19N (RHOA Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-EGFP-T2A-Bsd

Plasmid

#193198

Purpose

Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mBlueberry2-P2A-EGFP

Plasmid

#184937

Purpose

Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mBlueberry2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-EGFP-WPRE

Plasmid

#51504

Purpose

Can be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

EGFP

Use

AAV

Promoter

phSyn1

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO-EGFP-G3BP1-S149A

Plasmid

#136004

Purpose

DOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S149A

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO-EGFP-G3BP1-DeltaRBP

Plasmid

#136005

Purpose

DOX-inducible G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C-term of G3BP1 Deleted for Delta RBP

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

Plasmid

#113865

Purpose

Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Plasmid

#39761

Depositor

Pamela Raymond

Article

Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

Insert

zebrafish gfap promoter- EGFP (gfap Zebrafish)

Use

Zebrafish expression

Promoter

gfap

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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