We narrowed to 14,237 results for: cas9

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pCAG-Trex2-bpA-EF1BFP

Plasmid

#111144

Purpose

Expression of mouse Trex2 and BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

mouse Trex2 coding region (Trex2 Mouse)
BFP (Rnf112 )

Expression

Mammalian

Promoter

CAG and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pIW1

Plasmid

#52530

Purpose

serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Twin-STREP-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.RFP657

Plasmid

#69147

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-35S

Plasmid

#99861

Purpose

Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pG3H-U6EC1

Plasmid

#134758

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA-U6, zCas9-EC1

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS507-pCDest2-noAcr-mTagBFP2-IRES

Plasmid

#85748

Purpose

Expresses mTagBFP2 only; used as a control

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-PtH

Plasmid

#133391

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pRB20

Plasmid

#52559

Purpose

serves as PCR-template to generate HR donors for C-terminal YFP-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

eYFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pMiniT:myc-GluA1 TKIT donor

Plasmid

#169419

Purpose

Donor DNA for TKIT knockin of Myc to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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BII-C3HTK

Plasmid

#133388

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pG3K-U6EC1

Plasmid

#134757

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA-U6, zCas9-EC1

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pG3B-U6EC1

Plasmid

#134756

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA-U6, zCas9-EC1

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pG3H-U3Ub

Plasmid

#134749

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA- OsU3, zCas9-Ubi1

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-BtH

Plasmid

#133392

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pG3GB411

Plasmid

#134748

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA- OsU3, zCas9-Ubi1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAWp9-3

Plasmid

#73854

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg2

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
PABE-5

Plasmid

#115626

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

NLS-nCas9-NLS-wtTadA-TadA7.10-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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CP1300-CBEmax

Plasmid

#119805

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1300) -UGI-UGI

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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