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pTwist-SARS-CoV-2 Δ18 B.1.351v3

Plasmid

#169464

Purpose

Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S3-ExRaiAKAR

Plasmid

#160724

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter ExRai-AKAR (PKA indicator), V5 tag, and S3 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S3-ExRaiAKAR

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pME-mVenus-CAAX

Plasmid

#75153

Purpose

Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mVenus-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pVimentin-miRFP703

Plasmid

#79996

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Vimentin (VIM Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mCherry (JDW 1240)

Plasmid

#224499

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mCherry

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

Plasmid

#105201

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-IL12-P2A-CCL21-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

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FCK-Chronos-GFP

Plasmid

#80902

Purpose

Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with LFn + Fla

Plasmid

#84866

Purpose

Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Use

Baculovirus

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4-PSMA5-PSMA6-PSMA7

Plasmid

#214138

Purpose

Insect cell expression of human 20S CP alpha-subunits

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)
PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hMLH1dn-eGFP

Plasmid

#191104

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pKB225

Plasmid

#170015

Purpose

Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Peptide display protein with no C-terminal peptide

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-MCS

Plasmid

#122558

Purpose

Modified block 1, with deletion in E1

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 1-3759

Use

Synthetic Biology

Mutation

Adenovirus sequences 481-3530 replaced with multi…

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-QUASM1:GFPNLS-SV40pA

Plasmid

#155123

Purpose

Tol2 vector containing QUASM1 reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Zebrafish expression

Promoter

QUASM1-E1b

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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CD3-2A_pMI-LO

Plasmid

#153418

Purpose

Retroviral (MSCV) expression of all four WT CD3 subunits, co-expressed with LSSmOrange

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

murine CD3 delta-gamma-epsilon-zeta subunits (Cd3d Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP713

Plasmid

#136562

Purpose

F-actin labeling with a near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LifeAct-miRFP713

Tags

miRFP713

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

Plasmid

#123360

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

Empty LbCpf1 crRNA cloning cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

Plasmid

#104116

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

QuasAr3-Citrine

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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