We narrowed to 16,540 results for: puromycin

1. M76 pIRESpuro-GLUE-hDsh2

   Plasmid

   #15101

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   Dsh2 (DVL2 Human)
   

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-PCNA-IRES-puro2b

   Plasmid

   #26461

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   PCNA (PCNA Human)
   

   Tags

   pEGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRExT2A-NT-PmNG

   Plasmid

   #232200

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Expression

   Bacterial

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetO-FUW-BICRAL-FLAG-pgk-puro

   Plasmid

   #107732

   Purpose

   Tet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORF

   Depositor

   Emily Dykhuizen

   Article

   Alpsoy et al J Biol Chem. 2018 Jan 26. pii: RA117.001

   Insert

   BICRAL (BICRAL Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPV-EF1a-mRxxFP1(AfeI)-iP-A

   Plasmid

   #204625

   Purpose

   Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiPGK Puro DEST JNKKTRmCerulean3

   Plasmid

   #90232

   Purpose

   Lentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #106249

   Purpose

   Lentiviral vector with puro selection for expression of S. aureus dCas9-KRAB

   Depositor

   Charles Gersbach

   Article

   Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

   Insert

   dead S. aureus Cas9 KRAB T2A PuroR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-NLS and NLS-KRAB

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   hUbC

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. shMEK1/2 puro

   Plasmid

   #72567

   Purpose

   Hairpin targeting MEK1 and MEK2 (murine).

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   MEK1/2 (Map2k1, Map2k2 Mouse)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-Flag-CSNK1A1 WT-Puro

   Plasmid

   #123319

   Purpose

   Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid

    #86843

    Purpose

    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

    Depositor

    Thomas Cech

    Article

    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

    Insert

    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    

    Tags

    FLAG-HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous TERT promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSSA-RPG

    Plasmid

    #85932

    Purpose

    Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

    Depositor

    Zhiying Zhang

    Article

    Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

    Insert

    pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
    

    Use

    Dual-reporter surrogate

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-IRF4-Puro

    Plasmid

    #123323

    Purpose

    Lentiviral vector for expression of IRF4 under a Doxycycline inducible promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    Doxycycline inducible minimal CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe puro Snail

    Plasmid

    #23347

    Depositor

    Bob Weinberg

    Article

    Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

    Insert

    Snail (SNAI1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-puro Vps34-GFP

    Plasmid

    #38268

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. shMEK1/2 v2 puro

    Plasmid

    #72568

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

    Plasmid

    #221551

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-puro-miR-9 sponge

    Plasmid

    #25040

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    miR-9 sponge
    

    Use

    Retroviral

    Tags

    d2EGFP

    Expression

    Mammalian

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. BII-Sh-FUCCI-Luc

    Plasmid

    #133364

    Purpose

    Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Tags

    HA-tagged Clover-Geminin

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX459-TP53-exon4

    Plasmid

    #217455

    Purpose

    For TP53 knockout targeting exon 4 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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