We narrowed to 7,668 results for: CCH

1. ampl43

   Plasmid

   #161830

   Purpose

   pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK425 (GAL-OsTIR1(F74A))

   Plasmid

   #140657

   Purpose

   GAL-OsTIR1(F74A)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74A)
   

   Expression

   Yeast

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVtight-RFP-IRES-CD

   Plasmid

   #126678

   Purpose

   Doxycycline inducible expression of CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   RFP-IRES-CD (FCY1 Budding Yeast)
   

   Promoter

   tight TRE

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK198 (GAL-OsTIR1)

   Plasmid

   #140655

   Purpose

   GAL-OsTIR1(WT)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(WT)
   

   Expression

   Yeast

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. P1K-3HA-Atg8∆R-MET15

   Plasmid

   #207034

   Purpose

   Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆R

   Promoter

   pATG8

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS24

   Plasmid

   #58952

   Purpose

   Encodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.

   Depositor

   Thomas Fox

   Article

   Steele et al Proc Natl Acad Sci U S A. 1996 May 28;93

   Insert

   Mitochondrially coded Arg8 protein
   

   Mutation

   Coding sequence synthetic in S. cerevisiae mitoch…

   Promoter

   n/a

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pZac2.1 gfaABC1D-LifeAct-GFP

   Plasmid

   #190199

   Purpose

   Expresses the small actin binding peptide, LifeAct, with a GFP reporter

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   LifeAct-eGFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbA5c-MevT(CO)-MBIS(CO, ispA)

   Plasmid

   #35151

   Depositor

   Jay Keasling, Taek Soon Lee

   Article

   Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

   Insert

   AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   R364S in Mevalonate Kinase

   Promoter

   LacUV5,

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available Since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDS240

    Plasmid

    #34966

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Expression

    Yeast

    Mutation

    K533∆, high-affinity epitope

    Promoter

    GAL1

    Available Since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #78231

    Purpose

    Expresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistance

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Cas9
    

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFW2500

    Plasmid

    #224213

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1 and recT

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1 and recT
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV, lentiviral LTR

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTA-Mob 2.0

    Plasmid

    #149662

    Purpose

    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

    Depositor

    Bogumil Karas

    Article

    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

    Type

    Empty backbone

    Use

    Synthetic Biology; Conjugation helper plasmid

    Expression

    Bacterial

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. HDAC5 Flag

    Plasmid

    #13822

    Purpose

    Mammalian expression of human histone deacetylase 5 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC5 (HDAC5 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

    Plasmid

    #86420

    Purpose

    N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    CDC42pr-GFP1-10
    

    Tags

    GFP1-10

    Expression

    Yeast

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT010

    Plasmid

    #64762

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a 2xFLAG epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYES260-sc.eEF2

    Plasmid

    #85119

    Purpose

    yeast expression of EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT006

    Plasmid

    #64761

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, 2xFLAG to an HA-6xHIS epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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