59,541 results

1. pcDNA3-Flag-SP1

   Plasmid

   #232649

   Purpose

   expresses SP1 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   SP1 (SP1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0381

   Plasmid

   #142256

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMARCA2 (SMARCA2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

   Plasmid

   #218973

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Expression

   Mammalian

   Mutation

   mutant SRSF1 binding motif (AGTCTCCT)

   Promoter

   RSV LTR

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG426-GAL-Kar2-Beta-Amyloid

   Plasmid

   #181707

   Purpose

   Galactose inducible expression of Kar2-Beta-Amyloid

   Depositor

   Alejandro Chavez

   Insert

   Kar2-Beta-Amyloid
   

   Expression

   Yeast

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-ORP8-H514A-H515A

   Plasmid

   #208360

   Purpose

   Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   ORP8 (OSBPL8 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H514A-H515A

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-GRAB_CRFmut

   Plasmid

   #208656

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-DCX-TurboID-V5

   Plasmid

   #190740

   Purpose

   Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   doublecortin (DCX Human)
   

   Tags

   TurboID and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX2T-TEV CHMP1B 1-143

   Plasmid

   #108286

   Purpose

   Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-143

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZBTB4-donor

   Plasmid

   #176374

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZBTB4 (ZBTB4 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EWS-GFP

    Plasmid

    #164701

    Purpose

    Expression of EWS-GFP

    Depositor

    Cheryl Arrowsmith

    Insert

    EWSR1 (EWSR1 Human)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1A eIF4A3

    Plasmid

    #83035

    Purpose

    mammalian expression of eIF4A3

    Depositor

    Gideon Dreyfuss

    Article

    Chan et al RNA. 2004 Feb;10(2):200-9.

    Insert

    eIF4A3 (EIF4A3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pBABE-hygro CDK4 R24C

    Plasmid

    #11254

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    R24C

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus2-WTSTAT3

    Plasmid

    #123165

    Purpose

    Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF2249

    Plasmid

    #143184

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF12 (TCF12 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDFDuet-MKK6-EE p38alpha

    Plasmid

    #82719

    Purpose

    Expresses constitutively active human MKK6 and murine p38 alpha.

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Inserts

    MKK6 (MAP2K6 Human)
    p38alpha (Mapk14 Mouse)
    

    Expression

    Bacterial

    Mutation

    Ser207 to Glu, Thr211 to Glu

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3 Ras-LOCKR-S

    Plasmid

    #216496

    Purpose

    FRET-based reporter for Ras activity, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S
    

    Expression

    Mammalian

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0987

    Plasmid

    #142715

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF2 (KLF2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVC-FUS-R521C

    Plasmid

    #175067

    Purpose

    expresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Tags

    HA and mVenus + mCherry

    Expression

    Mammalian

    Mutation

    changed Arginin 521 to Cystein; mVenus: C-Termina…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. SOX17-tdTomato-EhB

    Plasmid

    #210467

    Purpose

    Donor plasmid for knock-in tdTomato into the human SOX17 locus

    Depositor

    Kai Wang

    Insert

    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-Ran(Q69L)

    Plasmid

    #42048

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Ran (RAN Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q69L

    Available Since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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