We narrowed to 13,793 results for: cdna
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Plasmid#213366PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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PRRP-DuET
Plasmid#213367PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTAFR-DuET
Plasmid#213368PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
QRFP-DuET
Plasmid#213372PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2RY8-DuET
Plasmid#213365PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NPS-DuET
Plasmid#213353PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUCNR1-DuET
Plasmid#213381PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
S1PR4-DuET
Plasmid#213375PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSTR1-DuET
Plasmid#213376PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTGER1-DuET
Plasmid#213370PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP-IFP2.0C
Plasmid#215752PurposeExpresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP-IFP2.0C
ExpressionMammalianPromoterCMVAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP_K299E_D333A-IFP2.0C
Plasmid#215753PurposeExpresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP_K299E_D333A-IFP2.0C
ExpressionMammalianPromoterCMVAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 N-2Strep
Plasmid#210376PurposeExpresses SARS-CoV-2 N protein fused with 2Strep in mammalian cellsDepositorAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(3G)-mCherry
Plasmid#206945PurposeMammalian expression of heterobifunctional SasG linkers (3G)DepositorInsertsfGFP-SaSasG (3G)-mCherry
TagsmCherry and sfGFPExpressionMammalianAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_35-345_eGFP
Plasmid#208881PurposeExpress eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.DepositorAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_5H_eGFP
Plasmid#208861PurposeExpress eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.DepositorInsertTLNRD1 5 helix bundle (TLNRD1 Human)
ExpressionMammalianMutationDeleted amino acid 142-273Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SEC61B-2Strep
Plasmid#210375PurposeExpresses SEC61B fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHDR-PMG (pXK-1650)
Plasmid#208858PurposeHDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-mNeonGreen
Plasmid#191192PurposeFull-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.DepositorInsertALIX (P801G) (PDCD6IP Human, Synthetic)
Tags6xHIS and mNeonGreenExpressionMammalianMutationPro 801 mutated to GlyPromoterCMVAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(7G)-mCherry
Plasmid#206949PurposeMammalian expression of heterobifunctional SasG linkers (7G)DepositorInsertsfGFP-SaSasG (7G)-mCherry
TagsmCherry and sfGFPExpressionMammalianAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(6G)-mCherry
Plasmid#206948PurposeMammalian expression of heterobifunctional SasG linkers (6G)DepositorInsertsfGFP-SaSasG (6G)-mCherry
TagsmCherry and sfGFPExpressionMammalianAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(5G)-mCherry
Plasmid#206947PurposeMammalian expression of heterobifunctional SasG linkers (5G)DepositorInsertsfGFP-SaSasG (5G)-mCherry
TagsmCherry and sfGFPExpressionMammalianAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(4G)-mCherry
Plasmid#206946PurposeMammalian expression of heterobifunctional SasG linkers (4G)DepositorInsertsfGFP-SaSasG (4G)-mCherry
TagsmCherry and sfGFPExpressionMammalianAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(2G)-mCherry
Plasmid#206944PurposeMammalian expression of heterobifunctional SasG linkers (2G)DepositorInsertsfGFP-SaSasG (2G)-mCherry
TagsmCherry and sfGFPExpressionMammalianAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMextli_577-620_AB
Plasmid#148337PurposeInsect Expression of DmMextli_577-520DepositorInsertDmMextli_577-520 (mxt Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMextli_577-640_AB
Plasmid#148338PurposeInsect Expression of DmMextli_577-640DepositorInsertDmMextli_577-640 (mxt Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-620_AB
Plasmid#148355PurposeBacterial Expression of DmMextli_577-620DepositorInsertDmMextli_577-620 (mxt Fly)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-640_AB
Plasmid#148360PurposeBacterial Expression of DmMextli_577-640DepositorInsertDmMextli_577-640 (mxt Fly)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-DmPABPC1_J
Plasmid#146643PurposeBacterial Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
ExpressionBacterialMutation4 silent mutations compared to the sequence geven…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA
Plasmid#194725Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFBDepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-CBS (H65R)
Plasmid#182924PurposeMammalian expression plasmid for human CBS mutant H65RDepositorInsertCystathionine Beta-Synthase (CBS Human)
TagseGFPExpressionMammalianMutationchanged histidine 65 to argininePromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo W42A
Plasmid#191399PurposeW42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo W42A
Tags3xFLAGExpressionMammalianMutationW42APromoterCMVAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2393-Tier1-PhCMV-p2a_fastFT
Plasmid#169530PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, fast maturation time
ExpressionMammalianPromoterPhCMVAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64
Plasmid#169597PurposeTier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).DepositorInsertdead S.pyogenes Cas9 - VP64 fusion
ExpressionMammalianPromoterPhCMVAvailable SinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL464
Plasmid#69881Purposea dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORFDepositorInsertCIRL (Cirl Fly)
UsePhic31-integration vectorAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2392-Tier1-PhCMV-p2a_medFT
Plasmid#169529PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, medium maturation time
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1046-Tier1-PhCMV-4xCox8-mRuby2
Plasmid#169537PurposeTier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)DepositorInsertPCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2380-Tier1-PhCMV-TM
Plasmid#169485PurposeTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).DepositorInsertPCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-2xFKBP (no insert) (pBS1139)
Plasmid#185322PurposeThe empty vector used for the FKBP experiments in our paperDepositorTypeEmpty backboneExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
BirA-Myc_N_FOXI1
Plasmid#167702PurposeExpresses FOXI1 in mammalian cellsDepositorAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only