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pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBA407

Plasmid

#85970

Purpose

UPRE-mCh-Ubc-Neo

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

mCherry
Neo

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMK277 (mClover-NeoR)

Plasmid

#72793

Purpose

C-terminal tagging with mClover using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mClover-Neo

Use

CRISPR

Tags

mClover

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt1(WT)

Plasmid

#86631

Purpose

Expresses mCherry-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha variant 2

Plasmid

#188362

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMK288 (mAID-Bsr)

Plasmid

#72826

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Bsr

Use

CRISPR

Tags

mAID

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTagBFP-Akt1

Plasmid

#86594

Purpose

Expresses TagBFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shTEAD1/3/4-1

Plasmid

#193672

Purpose

Constitutive lentiviral expression of TEAD shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

TEAD

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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DD-DamWT-IRES2-mCherry

Plasmid

#159602

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the wild-type allele of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamWT-IRES2-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-Spi1

Plasmid

#131597

Purpose

expression of mouse Spi1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Spi1 (Spi1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCOM-CD63-COM

Plasmid

#138350

Purpose

Expressing COM-CD63-COM fusion protein in mammalian cells

Depositor

Baisong Lu

Article

Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

Insert

COM-CD63-COM fusion protein

Use

CRISPR and Lentiviral

Tags

COM is fused to the N and C termini of human CD63

Expression

Mammalian

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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Cytosolic Syk Biosensor

Plasmid

#125729

Purpose

FRET biosensor for studying SYK activities in cells

Depositor

Yingxiao Wang

Article

Xiang et al Cell Mol Bioeng. 2011 Dec;4(4):670-677.

Insert

SYK biosensor (ZAP70 Synthetic)

Tags

6xHistine Tag at the N-terminal.

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shTEAD1/3/4-2

Plasmid

#193673

Purpose

Constitutive lentiviral expression of TEAD shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

TEAD

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch1 LNR

Plasmid

#188336

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

Plasmid

#62897

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV human IRS-1 CAAX

Plasmid

#11238

Depositor

Ronald Kahn

Article

Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

Insert

IRS-1 (IRS1 Human)

Tags

CAAX

Expression

Mammalian

Mutation

C-terminal prenylation motif makes IRS-1 localize…

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pHRSin_1G4 hTCRβ-SNAP

Plasmid

#187573

Purpose

Lentiviral expression of hTCR beta(1G4), and SNAP

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 hTCR beta, and SNAP tag (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin_1G4 TCRα

Plasmid

#187572

Purpose

Lentiviral expression TCR alpha(1G4)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

1G4 TCR alpha (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mActA-FKBP-mCherry

Plasmid

#210295

Purpose

ActuAtor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-FKBP-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

Plasmid

#62914

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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