162,717 results

1. pRGEB32

   Plasmid

   #63142

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ExRai-CKAR2

   Plasmid

   #236096

   Purpose

   Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   ExRai-CKAR2
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AbVec1.1-IGLC2-XhoI

   Plasmid

   #99575

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEmerald-Nesprin3-C-18

   Plasmid

   #54203

   Purpose

   Localization: Nuclear Membrane , Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Nesprin3 (SYNE3 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pYPQ132B2.0

   Plasmid

   #99888

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMEH31 Lamp2b HA

   Plasmid

   #168208

   Purpose

   Mammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-GFAP-hChR2(H134R)-mCherry

   Plasmid

   #27055

   Purpose

   AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

   Depositor

   Karl Deisseroth

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   GFAP

   Available since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbA6c_PsulA_sfGFP

    Plasmid

    #224587

    Purpose

    SOS response reporter

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PsulA

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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