We narrowed to 18,127 results for: Mut

1. pCAG-PDZD8HA_sh_resistant

   Plasmid

   #105006

   Purpose

   Mammalian Expression of PDZD8, sh resistant

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   PDZD8 (Pdzd8 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The sequence recognized by shPDZD8 (TRCN000043105…

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TALED_Right-ND1-1397N-UGI

   Plasmid

   #183899

   Purpose

   Expresses TALED_Right-ND1-1397N-UGI in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   DDCBE for ND1 mutation
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJAK112

   Plasmid

   #167280

   Purpose

   CodA-based mutagenesis vector for C. difficile

   Depositor

   Robert Fagan

   Article

   Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

   Type

   Empty backbone

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5/FRT/TO_NES_STf

   Plasmid

   #182001

   Purpose

   CMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   NES-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

   Plasmid

   #191676

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6x HIS and flag

   Expression

   Mutation

   D433N

   Promoter

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-HA-Nedd4(C744E)/2797

   Plasmid

   #11206

   Depositor

   Allan Weissman

   Article

   Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

   Insert

   Nedd4-1 (Nedd4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cysteine 744 mutated to glutamic acid

   Promoter

   Available Since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

7. hRIP1 HA GFP K45A

   Plasmid

   #41389

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP1 (RIPK1 Human)
   

   Use

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (K45A)

   Promoter

   CMV

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEF HaloTag-SYB2

   Plasmid

   #197282

   Purpose

   Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYB2 and HaloTag (Vamp2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q76V and F77W

   Promoter

   EF1alpha

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-UBE2E1 C131A in pcDNA3.1

   Plasmid

   #118214

   Purpose

   Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed cysteine 131 to alanine

   Promoter

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pegRNA-GBA-10

    Plasmid

    #199271

    Purpose

    pegRNA used to induce GBA (c. 1226 A > G) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    GBA 1226AtoG pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 6xHis(TEV)-Drp1(K38A)-StrepII

    Plasmid

    #174423

    Purpose

    Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K38A

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS NL4-3 envFS eGFP PR-D25A

    Plasmid

    #101334

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Protease D25A

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Use

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MARCKS(1-40)-paGFP

    Plasmid

    #18695

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    MARCKS(1-40) (MARCKS Human)
    

    Use

    Tags

    photoactivatable GFP

    Expression

    Mammalian

    Mutation

    A206K mutation in paGFP

    Promoter

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEM705 Gso (Ser)

    Plasmid

    #109353

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEB2-TagRFP-T

    Plasmid

    #103998

    Purpose

    Plasmid encoding TagRFP-T

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP-T
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-NLS-ZapCV2-ALA

    Plasmid

    #203665

    Purpose

    Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-Wnt4

    Plasmid

    #35910

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt4 (WNT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent Mutation C (492) --> T

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p35S-A3A-PBE

    Plasmid

    #119773

    Purpose

    Conversion of C to T in potato

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    CaMv 2x35S

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKL2013

    Plasmid

    #172182

    Purpose

    A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.

    Depositor

    Kan Wang

    Article

    McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. VinculinTS-A50I/I997A

    Plasmid

    #111829

    Purpose

    Vinculin tension sensor with point mutations affecting ability to bind talin and actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed vinculin alanine 50 to isoleucine and iso…

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGAL4-CREB S133A

    Plasmid

    #15222

    Depositor

    Ugo Moens

    Article

    Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

    Insert

    CREB S133A (Creb1 Rat)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Serine 133 mutated to Alanine

    Promoter

    Available Since

    June 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. A3A-Gam

    Plasmid

    #119769

    Purpose

    Conversion of C to T monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Fluorescent reporter for MOBE1-2

    Plasmid

    #219945

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TALED_Left-ND1-1397C-UGI

    Plasmid

    #183893

    Purpose

    Expresses TALED_Left-ND1-1397C-UGI in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    DDCBE for ND1 mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Fast-GCaMP-EF20

    Plasmid

    #52645

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF20
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D362N/D366N mutated from GCaMP3

    Promoter

    T7

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pAID 1.1C-T2A-Bsr

    Plasmid

    #105986

    Purpose

    To make an auxin inducible degron mutant

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Chromosome Res. 2017 Oct;25(3-4):253-260

    Insert

    T2A-Bsr
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQA-LP574

    Plasmid

    #222953

    Purpose

    Expresses R120A LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Use

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 120 to Alanine

    Promoter

    T7

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBS NL4-3 envFS eGFP IN-D116A

    Plasmid

    #101333

    Purpose

    pBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activity

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 envFS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Integrase D116A

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRRL-VinculinTS-A50I

    Plasmid

    #111831

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I) (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    changed vinculin alanine 50 to isoleucine

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SG3ΔENV CA M66I

    Plasmid

    #149687

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Capsid residue Met 66 to Iso (CA M66I)

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MAC_N_NFAC3

    Plasmid

    #167781

    Purpose

    Expresses NFAC3 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    NFAC3 (NFATC3 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

    Plasmid

    #211839

    Purpose

    Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    UBIQUITIN10

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191169

    Purpose

    Expression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:p53(R248Q).s2:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCAG-HIF1 alpha K532R

    Plasmid

    #21102

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

37. MSCVhygro-F-G9adSET

    Plasmid

    #41722

    Purpose

    Retroviral expression of human G9a mutant in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9adset (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LLhG-pHG165a E62K

    Plasmid

    #90051

    Purpose

    Constitutive expression of a chimeric LacI:GalR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

    Insert

    LLhG/E62K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E62K

    Promoter

    Iq

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. human p53-(1-393)-L344P

    Plasmid

    #24862

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Leucine 344 mutated to Proline

    Promoter

    Available Since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

40. MAC_N_NFIB

    Plasmid

    #167783

    Purpose

    Expresses NFIB in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    NFIB (NFIB Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge