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We narrowed to 9,959 results for: Met

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Showing: 5901 - 5920 of 9959 results

  5. pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

    Plasmid
    #174633
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Publication
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174633-plasmid-map-sequence-id-346989

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  6. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  7. Epac-S-H94

    Plasmid
    #170341
    Purpose
    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170341-plasmid-map-sequence-id-335921

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  8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid
    #129053
    Purpose
    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129053-plasmid-map-sequence-id-256238

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  9. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129036
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA8 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129036-plasmid-map-sequence-id-256165

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  10. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129029
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA1 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129029-plasmid-map-sequence-id-256133

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  17. pFLAG-CMV2-mMLL4fusion

    Plasmid
    #256014
    Purpose
    This plasmid expresses a mutant mini-version of human MLL4 (KMT2D) composed of a mutant PHD4-6 (1358–1572aa) bearing E1516A/E1517A/D1518A/E1544A and a SET-containing C-terminal region (4507–5537aa).
    Depositor
    Min Gyu Lee
    Publication
    Dhar et al Genes Dev. 2012 Dec 15;26(24):2749-62. d
    Insert
    mMLL4 fusion (KMT2D Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    PHD(PHD4-6) and SET(Catalytic domain) along with …
    Promoter
    CMV
    Available since
    June 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    256014-plasmid-map-sequence-id-513256

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  18. pLX304-KDM6A-M1190T/E1335D

    Plasmid
    #203581
    Purpose
    Expresses mutant version of KDM6A with increased oxygen affinity in mammalian cells.
    Depositor
    Abhishek Chakraborty, William Kaelin
    Publication
    Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222
    Insert
    KDM6A (KDM6A Human)
    Use
    Lentiviral
    Tags
    HA-tagged
    Expression
    Mammalian
    Mutation
    Changed Methionine 1190 Threonine and Glutamic Ac…
    Promoter
    CMV
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    203581-plasmid-map-sequence-id-466509

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  19. pNIA-CEN-FLAG-LANS1-Set2

    Plasmid
    #122002
    Purpose
    FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast
    Depositor
    Brian Kuhlman
    Publication
    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d
    Insert
    histone methyltransferase SET2 (SET2 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Set2 point mutations K538G, K539S, R549G, K550S, …
    Promoter
    ADH1
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    122002-plasmid-map-sequence-id-238610

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  20. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid
    #221447
    Purpose
    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus
    Depositor
    Daniel Durocher
    Publication
    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.
    Insert
    NFATC2IP (NFATC2IP Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    SPR degron: hGEM (1-110) fragment that is fused t…
    Promoter
    CMV
    Available since
    July 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221447-plasmid-map-sequence-id-437531

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Showing: 5901 - 5920 of 9959 results