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M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

Plasmid

#122147

Purpose

Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

FTH1 (FTH1 Human)

Use

Lentiviral

Tags

NLS-iLID-GFP

Expression

Mammalian

Promoter

SFFV

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLX313-TP53-WT

Plasmid

#118014

Purpose

Constitutive expression of wild-type human Tumor Protein p53 (TP53)

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-EGR1

Plasmid

#192913

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

EGR1 (EGR1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

Plasmid

#235086

Purpose

GFP splicing reporter with CORO1B intron

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L858R

Plasmid

#116276

Purpose

Lentiviral expression of EGFR L858R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

L858R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryBCL11B-W

Plasmid

#208758

Purpose

Lentiviral vector expressing mCherry fused with BCL11B cDNA

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B (BCL11B Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 R62D actin-3XNLS P2A mCherry

Plasmid

#58477

Purpose

Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Mutation

Changed Arginine 62 to Aspartic Acid

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-CD16-TLR4-HA

Plasmid

#246906

Purpose

Expresses CD16-TLR4 chimera in mammalian cells

Depositor

William Kaelin

Article

Lampson et al Cell. 2024 Apr 25;187(9):2209-2223.e16.

Insert

CD16-TLR4 (TLR4, FCGR3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

N terminus of CD16, C terminus of TLR4

Promoter

CMV

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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SFFV-BMI1-Brd 494

Plasmid

#219388

Purpose

Transcription factor BMI1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

BMI1 (BMI1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 actin-3XNLS P2A mCherry

Plasmid

#58475

Purpose

Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Inserts

beta actin (ACTB Human)
3XNLS
P2A cleavage site

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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TFORF3412

Plasmid

#144888

Purpose

Lentiviral vector for overexpressing the EGR1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EGR1 (EGR1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-EGFP-GPI-stop-L2

Plasmid

#186355

Purpose

Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Synthetic, Human)

Use

Expression of a fluorescent membrane marker

Tags

EGFP

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR2

Plasmid

#116741

Purpose

Lentiviral expression of FGFR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR2 (FGFR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald

Plasmid

#164210

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Promoter

EF1a

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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Human IFN-gamma promoter/enhancer

Plasmid

#17598

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter with 1st intron enhancer (IFNG Human)

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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mCherry-APP-mGFP

Plasmid

#196704

Purpose

Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

Depositor

Martino Calamai

Article

Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

Insert

Amyloid Precursor Protein 695 isoform (APP Human)

Tags

mCherry and mEGFP

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_BMP2_WT

Plasmid

#82101

Purpose

Gateway Donor vector containing BMP2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BMP2 (BMP2 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-human Erbb2-IRES-GFP

Plasmid

#91888

Purpose

Retroviral expression of human Erbb2

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Erbb2 (ERBB2 Human)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Promoter

MMLV gag

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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