We narrowed to 30,707 results for: PLE
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Plasmid#24295DepositorAvailable SinceAug. 27, 2010AvailabilityAcademic Institutions and Nonprofits only
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pET11-His6-Pif6-sfGFP-ssrA
Plasmid#140891PurposeExpression of PIF-sfGFP-ssrA in E. coliDepositorInsertPIF-sfGFP-ssrA
ExpressionBacterialPromoterT7Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-CAG-hrGFP
Plasmid#52344PurposeAAVS1 donor vector for constitutive gene expressionDepositorInserthrGFP
ExpressionMammalianPromoterCAGAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR
Plasmid#118405PurposecpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR
ExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b-RvLEAMshort
Plasmid#218122PurposeExpresses truncated RvLEAM protein under IPTG induction in bacterial host cells.DepositorInsertRvLEAMshort
Tags6xHISExpressionBacterialMutationTruncated form (58-181aa)PromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFD4-U6:1_U6:3tandemgRNAs
Plasmid#49411Purposeexpression of two gRNAs from Drosophila U6:1 and U6:3DepositorInsertsdU6-1:gRNA
dU6-3:gRNA
UseCRISPRExpressionInsectPromoterdU6-1 and dU6-3Available SinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FMRP_P451S
Plasmid#198693PurposeExpression of human FMRP protein in E. coliDepositorInsertFMR1 (FMR1 Human)
TagsMBPExpressionBacterialMutationchanged proline 451 to serinePromoterT7Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCR5-donor-MCS
Plasmid#192269PurposeDonor template for CRISPR Genome EditingDepositorInsertMCS within donor template
UseCRISPRAvailable SinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Left
Plasmid#52341PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Left
ExpressionMammalianPromoterCMVAvailable SinceJuly 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 TALEN Right
Plasmid#52342PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Right
ExpressionMammalianPromoterCMVAvailable SinceJuly 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/CyclinB1-GFP (28)
Plasmid#39871DepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pAGM47523
Plasmid#153221PurposeLevel 0 zCas9i vectorDepositorInsertSpCas9 with introns and 2 NLS
UseSynthetic BiologyAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
MS2-epegRNA-Dnmt1
Plasmid#211372PurposeDnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP productionDepositorInsertMS2-epegRNA
ExpressionMammalianAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-EV2.1
Plasmid#158411PurposeCRISPR-dpcoCas9 fused with EDLL activator for transcriptional activationDepositorInsertdpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
UseCRISPRExpressionPlantAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCk3
Plasmid#136697PurposeAcceptor plasmid for odd level position 3, lacZ blue-white screening.DepositorInsertlacZ
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV5B-TGFbeta receptor I K232R
Plasmid#11763DepositorInsertTGFbeta receptor I (TGFBR1 Human)
Tags6xHISExpressionMammalianMutationK232R: kinase deadPromoterCMVAvailable SinceMay 12, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro FLAG SREBP2
Plasmid#32018DepositorAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
L1_CsR-Ck1
Plasmid#136124PurposeL1 in position 1 (next to LB in L2 construct), for CsRDepositorInsertp5-35Sx2:mALS_3t35S
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
8xNFAT-ZsG-mCD8
Plasmid#162747PurposeNFAT-driven ZsGreen-1 reporter gene with mouse CD8 geneDepositorInsert8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
UseRetroviralExpressionMammalianAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianPromoterhUbCAvailable SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
ExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
2xMyc-tdMCP-SNAP (tdMCP standard)
Plasmid#226166PurposeFor transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.DepositorInsert2xMyc-tdMCP-SNAPtag
UseLentiviralTags2xMyc-tdMCP and EGFP-IRES-BlastRExpressionMammalianAvailable SinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCk3_spacer
Plasmid#136073PurposeUse when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits