We narrowed to 13,747 results for: Cre

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Nsp3 Mac1 - P43 (Oxford)

Plasmid

#169210

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Publication

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMTU-DO-URA

Plasmid

#160004

Purpose

Low-copy expression vector for K. marxianus, uracil selection

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

n/a

Expression

Yeast

Promoter

n/a

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1

Plasmid

#109581

Purpose

Multisite gateway vector for 3' tagging with Rac1

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-HA-Nedd8-P2A-Hygro

Plasmid

#124306

Purpose

Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Publication

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

Nedd8 (NEDD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pOCC72

Plasmid

#118866

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Publication

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6, cleavable with 3C protease, mGFP, cleavable…

Expression

Insect

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG-FGFRdn

Plasmid

#80431

Purpose

Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

Depositor

Aixa Morales

Publication

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Fibroblast growth factor receptor 1 (FGFR1 Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1–425

Promoter

beta-actin promoter

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
mCitrine-USP19 C506S

Plasmid

#78594

Purpose

Express mCitrine-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 C506S (USP19 Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
pETpRha-alpha-SkTm

Plasmid

#72929

Purpose

Allows expression of rabbit alpha-skeletal tropomyosin from a rhamnose inducible promoter

Depositor

Dan Mulvihill

Publication

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha skeletal Tropomyosin

Expression

Bacterial

Promoter

rhaB

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-Cx43

Plasmid

#60691

Purpose

mammalian expression of rat Cx43

Depositor

Parmender Mehta

Publication

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt2-V5

Plasmid

#35925

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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CXCR2-DuET

Plasmid

#213219

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Publication

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CXCR2 (CXCR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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AWP-029

Plasmid

#213966

Purpose

Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for gRNA constitutive expression.

Depositor

Alexander Westermann

Publication

Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

Insert

Cas12a (cas12a Synthetic, Segatella bryantii)

Tags

3XFLAG

Expression

Bacterial

Mutation

D875A to inactivate target nuclease activity

Promoter

PcfxA, IPTG inducible

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-idCas9-KRAB-Hygro donor

Plasmid

#199621

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Publication

Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

Insert

AAVS1-idCas9-KRAB-Hygro

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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CBP TAZ1(340-439)

Plasmid

#173760

Purpose

bacterial expression of mouse CBP TAZ1 domain

Depositor

Peter Wright

Publication

De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

Insert

CBP TAZ1 (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1-DN

Plasmid

#109582

Purpose

Multisite gateway vector for 3' tagging with dominant negative Rac1

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Plxnb2(S)-Fc-His

Plasmid

#72129

Purpose

Expresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Sema4a-Fc-His

Plasmid

#72154

Purpose

Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Publication

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4a (Sema4a Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-EGFP_puromycin

Plasmid

#65260

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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ManII-SBP-mCherry_puromycin

Plasmid

#65261

Purpose

ManII reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

truncated Mannosidae II

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Publication

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article